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| 1 | Probiotic metabolites from Bacillus coagulans GanedenBC30^(TM) support maturation of antigen-presenting cells in vitro显示文摘AIM:To study the effects of probiotic metabolites on maturation stage of antigen-presenting immune cells.METHODS:Ganeden Bacillus coagulans 30(GBC30) bacterial cultures in log phase were used to isolate the secreted metabolite(MET) fraction.A second fraction was made to generate a crude cell-wall-enriched fraction,by centrifugation and lysis,followed by washing.A preparation of MET was subjected to size exclusion centrifugation,generating three fractions:< 3 kDa,3-30 kDa,and 30-200 kDa and activities were tested in comparison to crude MET and cell wall in primary cultures of human peripheral blood mononuclear cell(PBMC) as a source of antigen-presenting mononuclear phagocytes.The maturation status of mononuclear phagocytes was evaluated by staining with monoclonal antibodies towards CD14,CD16,CD80 and CD86 and analyzed by flow cytometry.RESULTS:Treatment of PBMC with MET supported maturation of mononuclear phagocytes toward both macrophage and dendritic cell phenotypes.The biological activity unique to the metabolites included a reduction of CD14+ CD16+ pro-inflammatory cells,and this property was associated with the high molecular weight metabolite fraction.Changes were also seen for the dendritic cell maturation markers CD80 and CD86.On CD14dim cells,an increase in both CD80 and CD86 expression was seen,in contrast to a selective increase in CD86 expression on CD14bright cells.The co-expression of CD80 and CD86 indicates effective antigen presentation to T cells and support of T helper cell differentiation.The selective expression of CD86 in the absence of CD80 points to a role in generating T regulatory cells.CONCLUSION:The data show that a primary mechanism of action of GBC30 metabolites involves support of more mature phenotypes of antigen-presenting cells,important for immunological decision-making. | Kathleen F Benson Kimberlee A Redman Steve G Carter David Keller Sean Farmer John R Endres Gitte S Jensen | 2012 | World Journal of Gastroenterology2012，18，16： | 8 |
| 2 | An analytic approach to practical state space reconstruction 显示文摘 | Gibson J F Farmer J Casdagli M | 1992 | Physica D1992，57，： | 1 |
| 3 | Timing of volcanism in the Sierra Nevada of California:Evidence for Pliocene delamination of the batholithic root?显示文摘 | Manley C R Glazner A F Farmer G L | 2000 | Geology2000，28，： | 1 |
| 4 | Oligosaccharide signals in plants: A current assessment 显示文摘 | Ryan C A Farmer E F | 1991 | Annu Rev Phy-Siol Mol Biol1991，42，651： | 1 |
| 5 | Enterobacter Skazakii:A New Species of 'Enterbacterlaceae'lsolated from Clinical Deci rrlerls显示文摘 | FARMER J J ASBURY M A HICKMAN F W | 1980 | Int H Syst Bacterlol1980，30，： | 1 |
| 6 | Did lithospheric delamination trigger late Cenozoic potassic volcanism in the southern Sierra Nevada,California?显示文摘 | Farmer G L Glazner A F Manley C R | 2002 | Geological Society of America Bulletin2002，114，： | 1 |
| 7 | Advances in pediatric liver and intestinal transplantation 显示文摘 | Ghobrial RM Farmer DG Amersi F | 2000 | Am J Surg2000，180，5： | 1 |
| 8 | Graphene field-effect tran- sistors with high on/off current ratio and large transport band gap at room temperature显示文摘 | Xia F N Farmer D B Lin Y M | 2010 | Nano Lett2010，10，2： | 1 |
| 9 | An analytic approach to practical state space reconstruction显示文摘 | Jone F Gibson Doyne Farmer J | | 0，，： | 1 |
| 10 | CD62 blockade with P-Selectin glycoprotein ligand-immunoglobulin fusion protein reduces ischemia-reperfusion injury after rat intestinal transplantation显示文摘 | FARMER D G SHEN X D AMERSI F | 2005 | Transplantation2005，79，1： | 1 |
| 11 | L Calcinurin is a comnon target of cydophilin-cycloxporin A and FKBp-FKS06 complexes显示文摘 | Liu J Farmer JD Lane WS F | 1991 | Cell1991，66，： | 1 |
| 12 | Hormonal signaling and transcriptional control of adipocyte differentiation 显示文摘 | MORRISON R F FARMER S R | 2000 | The Journal of Nutrition2000，130，12： | 1 |
| 13 | Subsurface filamentous fabrics: An evaluation of origins based on morphological and geochemical criteria, with implications for exopaleontology 显示文摘 | Hofmann B A Farmer J D yon Blanckenburg F | 2008 | Astrobiology2008，8，： | 1 |
| 14 | Organic Antimony Compounds as Lubricant Additives显示文摘 | H H Farmer B W Malone H F Tompkins | 1967 | Lubrication Engineering1967，23，3： | 1 |
| 15 | Asbury M A Biochemical identification of new species and biogroups of Enterobacteriaceae isolated from clinical specimens显示文摘 | Farmer Ⅲ J J Davis B R Hickman-Brenner F W | 1985 | Clin Microbiol1985，21，： | 1 |
| 16 | A Psychometric Evaluation of the Revised Temperament and Character Inventory (TCI - R) and the FCI - 140显示文摘 | ichard F Farmer Lewis R | 2008 | Psychological Assessment2008，20，3： | 1 |
| 17 | Biochemi- cal identification of new species and biogroups of enterobaete- riaceae isolated from clinical speeimens显示文摘 | Farmer J J Davis B R Hickman-Brenner F W | 1985 | J Clin Mierobiol1985，21，1： | 1 |
| 18 | Community- Based Therapy for Multidrug-Resistant Tuberculosis in Lima, Peru显示文摘 | Mitnick C Bayona J Palacios E Shin S Furin J Alcantara F Sdnchez E Sarria M Becerra M Fawzi MC Kapiga S Neuberg D Maguire JH Kim JY Farmer P | 2003 | N EnglJ Med2003，348，2： | 1 |
| 19 | Destruction of chlorinated organics by cobalt(Ⅲ)-mediated electrochemical oxidation显示文摘 | Farmer J C Wang F T Lewis P R | 1992 | J Electrochem Soc1992，139，11： | 1 |
| 20 | Assay validation for KIM-I: human urinary renal dysfunction biomarker显示文摘 | CHATURVEDI S FARMER T KAPKE G F | 2009 | Int J Biol Sei2009，5，2： | 1 |
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