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| 1 | Alterations in metastatic properties of hepatocellular carcinoma cell following H-ras oncogene transfection显示文摘AIM To demonstrate the relationship betweenH-ras oncogene and hepatocellular carcinoma(HCC) metastasis.METHODS Activated H-ras oncogene wastransfected into SMMC 7721, a cell line derivedfrom human HCC, by calcium phosphatetransfection method. Some metastasis-relatedparameters were detected in vitro, includingadhesion assay, migration assay, expression ofcollagenase ⅣV (c ⅣV ase) and epidermal growthfactor receptor (EGFR).RESULTS The abilities of H-ras-transfected cellclones in adhesion to laminin (LN) or fibronectin(FN), migration, c Ⅳ ase secretion increasedmarkedly, and the expression of EGFR elevatedmoderately. More importantly, these alterationswere consistent positively with the expressionof p21, the protein product of H-ras oncogene.CONCLUSION H-ras oncogene could inducethe metastatic phenotype of HCC cell in vitro toraise its metastatic potential. | Qing Wang~1 Zhi Ying Lin~2 Xiao Li Feng~3 ~1Department of Microbiology,Medical Center of Fudan University.the former Shanghai Medical University,Shanghai 200032,China ~2Liver Cancer Institute,Zhongshan Hospital,Shanghai 200032,China ~3Shanghai Institute of Biochemistry,Academy Sinica,Shanghai 200031,ChinaQing Wang earned master degree from Shanghai Medical University in 1996,now a senior lecturer of microbiology,specialized in the role of oncogcncs on tumor metastasis,having 8 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 48 |
| 2 | Antihepatoma effect of alpha-fetoprotein antisense phosphorothioate oligodeoxyribonucleotides in vitro and in mice显示文摘AIM To evaluate antihepatoma effect ofantisense phosphorothioate oligodeo-xyribonucleotides (S-ODNs) targeted to alpha-fetoprotein (AFP) genes in vitro and in nudemice.METHODS AFP gene expression was examinedby immunocytochemical method or enzyme-linked immunosorbent assay. Effect of S-ODNson SMMC-7721 human hepatoma cell growth invitro was determined using microculturetetrazolium assay. In vivo antitumor activitiesof S-ODNs were monitored by measuring tumorweight differences in treated and control micebearing SMMC-7721 xenografts. Induction of cellapoptosis was evaluated by fluorescence-activated cell sorter (FACS) analysis.RESULTS Antisense S-ODN treatment led toreduced AFP gene expression. Specificantisense S-ODNs, but not control S-ODNs,inhibited the growth of heaptoma cells in vitro.In vivo. only antisense S-ODNs exhibitedobvious antitumor activities. FACS analysisrevealed that the growth inhibition by antisenseS. ODNs was associated with their cell apoptosisinduction.CONCLUSION Antisense S-ODNs targeted toAFP genes inhibit the growth of human hepatomacells and solid hepatoma, which is related totheir cell apoptosis induction. | Xing Wang Wang~1 Jin Hui Yuan~1 Ru Gang Zhang~1 Li Xia Guo~1 Yong Xie~2 Hong Xie~1 ~1Department of Biotherapy,Shanghai Institute of Cell Biology,Chinese Academy of Sciences,Shanghai 200031,China ~2Department of Biology,Hong Kong University of Science and Technology,ChinaDr.Xing Wang Wang earned Ph.D.from Shanghai Institute of Materia Medical,Chinese Academy of Sciences in 1997.Now a professor at Shanghai Institute of Cell Biology,Chinese Academy of Sciences. | 2001 | World Journal of Gastroenterology2001，7，3： | 21 |
| 3 | Orthotopic transplantation model of human gastrointestinal cancer and detection of micrometastases显示文摘AIM To establish a relevant animal model ofhuman gastrointestinal cancer, which can beused for repetitive investigations, so as toimprove our understanding and management ofcarcinogenesis and cancer metastasis.METHODS Intact tissues of human colorectaland pancreatic cancers were transplanted innude mice. The biological characteristics of theoriginal and the corresponding transplantedtumors were investigated by HE staining, PASstaining and immunostaining. The metastases inthe livers and lungs of nude mice wereinvestigated by immunostaining withbiotinylated mab KL-1 and by RT-PCR using CK20specific primers.RESULTS There were totally 9 of 16 surgicalspecimens growing in nude mice subcutaneouslyand/.or orthotopically (4 of 6 colorectal and 5 of10 pancreatic cancer). Tumor cell content of thespecimens and freezing of tissue specimens areimportant factors influencing the growth oftransplanted tumor. In the group of fresh tumortissues with greater than 50% tumor cellcontent, the success rate of the transplantationwas 100% (3 cases of pancreatic cancer and 3cases of colorectal cancer). The orthotopicallytransplanted tumors resemble the original tumormorphologically and biologically, including TAAexpression such as CEA byimmunohistochemistry, and CEA level in theserum of mice. Ki-67 labeling index and theexpression of TAA especially K-ras, 17-lA andRA-96, are associated with the potential of tumorgrowth in nude mice. Micrometastases in thelungs and livers of tumor bearing mice can bedetected by immunostaining with biotinylatedmab KL-1 and CK20-specific RT-PCR.CONCLUSION An orthotopic transplantationmodel for human colon and pancreatic cancer innude mice has been set up. We have alsoestablished sensitive detection methods withCK-immunohistochemistry and CK20-RT-PCR tostudy xenotransplanted human cancer and itsmetastatic cancer cells in the liver and lung ofnude mice. This study may be helpful inunderstanding the mechanism of cancermetastasis and in developing new diagnosticmethods and therapeutic strategies formetastases including micrometastases. | Jun Hui Cui~1 Uwe Krueger~2 Doris Henne-Bruns~2 Bemd Kremer~2 Holger Kalthoff~2 ~1Department of General Surgery,First Affiliated Hospital,College of Medicine,Zhejiang University,Hangzhou 310003,Zhejiang Province,China ~2Department of General Surgery,Christian-Albrechts-University,Kiel,GermanyDr.Jun Hui Cui graduated from Zhejiang Medical University in 1984,earned master degree in 1990,studied in the Surgical Department of Kiel University and worked in the Lab of Molecular Oncology of Kiel University from 1994-1997achieved M.D.from Kiel University.Germany,now associate professor of surgery,specialized in colorectal oncology.Adviser of graduated student for master degree,having 20 publications published in key Chinese or English journals. | 2001 | World Journal of Gastroenterology2001，7，3： | 19 |
| 4 | 知识型企业领导成员交换与知识共享的关系研究显示文摘在分析领导成员交换关系与知识共享的相互作用基础上,构建了领导成员交换与知识共享的关系模型,采用多元统计分析方法对知识型企业领导成员交换与知识共享的相互关系进行了实证分析。研究结果表明:领导成员交换和知识共享之间存在显著的正相关关系,而且体现出维度差异,领导成员交换的沟通、信任维度可以较好地由知识共享典型变量来预测。 | 张莉 贾琼 刘宝巍 brian earn | 2008 | 科学学研究2008，26，S2： | 10 |
| 5 | 基于领导成员交换关系的沟通满意度研究显示文摘沟通满意度作为组织有效沟通的一个重要评价指标,对提高员工的工作积极性、工作效率,增加员工对组织的责任感起着重要作用。本研究基于领导成员交换理论,分析了领导成员交换关系与沟通满意度的相关变量,并构建了沟通满意度与领导成员交换的关系模型。在此基础上,通过调查研究,采用多元统计分析方法对沟通满意度与领导成员交换关系进行了实证研究,研究结果表明:沟通满意度与领导成员交换有正相关关系;沟通满意度中领导的下行沟通比成员的上行沟通对领导成员交换关系的影响更强;领导成员交换质量将影响个体、团队和组织层次上的沟通满意度。 | 张莉 刘宝巍 贾琼 Brian Earn | 2009 | 管理评论2009，21，4： | 10 |
| 6 | Chemotherapy with or without radiation therapy in limited small-cell carcinoma of the lung显示文摘 | Perry M C Earn W L Propert K J | 1987 | N Engl J Med1987，316，15： | 1 |
| 7 | Opposite patterns of synchrony in sympatric disease metapopulations显示文摘 | Rohani P Earn DJD Grenfell BT | 1999 | Science1999，286，5441： | 1 |
| 8 | Aortic arch calcification and vascular disease: the Guangzhou Biobank Cohort Study 显示文摘 | Xu L Jiang CQ Earn TH | 2010 | Cardiology2010，117，4： | 1 |
| 9 | Vaccination and the theory of games显示文摘 | BAUCH C T EARN D J D | 2004 | Proe Natl Aead Sci USA2004，，101： | 1 |
| 10 | Group interest versus self-interest in smallpox vaccination policy显示文摘 | BAUCH C T GALVANI A P EARN D J D | 2003 | Proceedings of the National Academy of Sciences2003，100，10： | 1 |
| 11 | Group interest versus self-interest in smallpox vaccination policy 显示文摘 | Bauch C T Galvani A P Earn D J D | 2003 | Proc Natl Acad Sci USA2003，100，10： | 1 |
| 12 | Effects of school closure on incidence of pandemic influenza in Alberta, Canada 显示文摘 | Earn DJ He D Loeb MB | 2012 | Ann InternMed2012，156，3： | 1 |
| 13 | Vaccination and the theory of games 显示文摘 | Bauch C T Earn D J D | 2004 | Proc Natl Acad Sci USA2004，101，13： | 1 |
| 14 | Ecology and evolution of the flu显示文摘 | Earn D J D Dushoff J Levin S A | 2002 | Trends Ecol Evol2002，17，7： | 1 |
| 15 | Lane detection and tracking using BOSnake 显示文摘 | WANG Yue SHEN Ding-gang KHWANG Teoh Earn | 2004 | Image Vi sion Computation2004，22，4： | 1 |
| 16 | Book Values, and Divi-dends in Equity Valuation显示文摘 | OHLSON J EARNING S | 1995 | Contemporary Ac counting Research1995，11，2： | 1 |
| 17 | Mice deficient in stem cell antigen-1 (Scal, Ly-6A/E) develop normal prinmry and memory CD4+ and CD8+ T-cell responses to virus infection 显示文摘 | Whitmire J K Earn B Whitton J L | 2009 | Eur J Immunol2009，39，6： | 1 |
| 18 | Jounal of Accounting &Economics显示文摘 | Basu S The conservatism principle and the Asymmetri cTimelin ess of Earnings | 1997 | (1)1997，，1： | 1 |
| 19 | Lane detection and tracking using B-Snake 显示文摘 | Yue Wang Earn Khwang Teoh Dinggang Shen | 2003 | Image and Vision Computing2003，224，： | 1 |
| 20 | Vaccination and the theory of games显示文摘 | BAUCH C T EARN D J D | 2004 | Proceedings of the National Academy of Sciences of the United States of America2004，101，13： | 1 |
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