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| 1 | Semen analysis in 21st century medicine: the need for sperm function testing显示文摘一旦通常在采用帮助繁殖技术(艺术) 前为不肥沃的夫妇表现了,精子功能测试在全世界从宠爱掉在许多繁殖的药中心里。确实,最近的增加“世界健康组织() 为考试用手、人的精液处理的实验室”现在这些组织许多进节的过程称为研究过程。在大部分,当假定那时,如果一个精子能被作出对有利的裁决,这反映绕过男搭挡的深入的评估的当前的临床的实践 intracytoplasmic 精子注射(ICSI ) ,它一定是能够完成授精的一个健康房间。不过,精子功能测试能提供珍贵临床的卓见进引起男不孕的缺点。在一些情况中,无可否认地,功能的精子缺乏能用艺术被克服。在另外的情况中,夫妇们将被他们的不孕的原因的知识授权,并且也许为一些联合甚至 ICSI 成功的可能性的(相对精子) 。知识允许他们使繁殖决定真正明智,包括(也许) 寻求施主授精,以采用或仍然保持无儿女的决定。他们的不孕的原因的知识可以关于他们繁殖处理的选择为夫妇和信心的一种感觉提供闭合。 | Dolores J. Lamb | 2010 | Asian Journal of Andrology2010，12，1： | 25 |
| 2 | Endurance exercise and gut microbiota:A review显示文摘Background: The physiological and biochemical demands of intense exercise elicit both muscle-based and systemic responses. The main adaptations to endurance exercise include the correction of electrolyte imbalance, a decrease in glycogen storage and the increase of oxidative stress, intestinal permeability, muscle damage, and systemic inflammatory response. Adaptations to exercise might be influenced by the gut microbiota, which plays an important role in the production, storage, and expenditure of energy obtained from the diet as well as in inflammation,redox reactions, and hydration status.Methods: A systematic and comprehensive search of electronic databases, including MEDLINE, Scopus, Clinical Trials.gov, Science Direct,Springer Link, and EMBASE was done. The search process was completed using the keywords: 'endurance', 'exercise', 'immune response','microbiota', 'nutrition', and 'probiotics'.Results: Reviewed literature supports the hypothesis that intestinal microbiota might be able to provide a measureable, effective marker of an athlete's immune function and that microbial composition analysis might also be sensitive enough to detect exercise-induced stress and metabolic disorders. The review also supports the hypothesis that modifying the microbiota through the use of probiotics could be an important therapeutic tool to improve athletes' overall general health, performance, and energy availability while controlling inflammation and redox levels.Conclusion: The present review provides a comprehensive overview of how gut microbiota may have a key role in controlling the oxidative stress and inflammatory responses as well as improving metabolism and energy expenditure during intense exercise. | Núria Mach Dolors Fuster-Botella | 2017 | Journal of Sport and Health Science2017，6，2： | 18 |
| 3 | Epidemiological Evolution of Lung Cancer in the South of Spain from 1990 to 2010显示文摘Background: Changes in lung cancer has been characterized by the increase of cases among women and the increase in adenocarcinomas among other histological subtypes.Methods: Descriptive analysis of cases diagnosed with lung cancer in Hospital Virgen de las Nieves(Spain) from 1990 to 2010,based on five variables(age, sex, smoking, histology and pathological anatomy). The study establishes associations between these variables and compares the results with the literature.Results: 2,026 patients were diagnosed with lung cancer in this period; 1,838 were males(90.7%) and 188 women(9.3%); 1,892 patients(93.4%) were smokers or ex-smokers and 134(6.6%) had never smoked; the most frequent non-small cell histology types were squamous cell carcinoma and adenocarcinoma and it was the most frequent neoplasia in women and were associated with a lower tobacco consumption.Conclusion: The large majority of lung cancer cases is associated with a history of smoking tobacco and there are histopathological differences according to gender and cumulative tobacco smoke load. | Alberto Caballero Vázquez Ana Dolores Romero Ortiz Jose Manuel González de Vega San Román Raimundo García del Moral Bernardino Alcázar Navarrete | 2018 | 中国肺癌杂志2018，21，1： | 17 |
| 4 | Participation of epididymal cysteine-rich secretory proteins in sperm-egg fusion and their potential use for male fertility regulation显示文摘老鼠蛋白质 DE 是一个雄激素依赖者充满半胱氨酸的能分泌的蛋白质(松脆) 由近似附睾区域综合了。DE,也作为 CRISP-1 知道,在反应 acrosome 的精子的赤道的片断上是局部性的并且通过绑定参予配偶子熔化到鸡蛋互补地点。有 DE 的老鼠的免疫禁止富饶和精子熔化能力,建议 DE 代表一个好附睾避孕药目标。Recombinant 整理碎片和合成的肽表明 DE 经由 anevolutionarily 保存的主题的一个 12-amino 酸区域绑在卵,签名 2 (S2 ) 。松脆的其它的能力与他们的 S2 氨基酸序列被相关绑在老鼠卵。尽管阴囊的蛋白质 Tpx-1 (CRISP-2 ) 能够到啮齿类动物卵的绑定,人的附睾的 AEG 相关的蛋白质(ARP ) 和 helothermine (从蜥蜴口水) 不。S2 区域在 Tpx-1 介绍了仅仅二替换并且四在 ARPand helothermine,与 DE S2 相比,建议这氨基酸定序为鸡蛋是相关的相互作用。有 Tpx-1 和 anti-Tpx-1 的研究在卵通过绑定在配偶子熔化揭示了这蛋白质的参予到互补地点。在比赛研究, DE dose-dependently 减少了 Tpx-1 的绑定,显示那两个松脆的份额卵互补地点。当认出仅仅相应蛋白质时,那 anti-DE 和 anti-Tpx-1 禁止精子鸡蛋熔化,建议在这些之间的功能的合作相应对松脆保证授精成功。这些结果增加我们配偶子熔化的分子的机制的理解并且贡献调整方法的新、更安全的富饶的发展。 | Debora J. Cohen Vanina G. Da Ros Dolores Busso Diego A. Ellerman Julieta A. Maldera Nadia Goldweic Patricia S. Cuasnicti | 2007 | Asian Journal of Andrology2007，9，4： | 16 |
| 5 | The varicocele: diagnostic dilemmas, therapeutic challenges and future perspectives显示文摘精索静脉曲张在精索以内被定义为内部阴囊的静脉和 pampiniform venus 丛的反常膨胀。如果精液分析没从青春期的男性被获得,当另外的症状不在时,主要临床的指示由许多泌尿科医师使用了建议修理是阴囊的萎缩。精索静脉曲张可以在与损害精子引起阴囊的萎缩的一些男性导致阴囊的损坏生产和减少的 Leydig 房间工作,当在另外的男性精索静脉曲张可以看起来不引起有病的效果时。在成年的人,精索静脉曲张经常在场、通过手术可改正,还外科的修理的可测量的好处根据 Cochrane 评论是细微的。当比肥沃的人更通常发生在不肥沃的人时,仅仅,有记录精索静脉曲张的 20% 人将受不了富饶问题。在青少年发现的大多数精索静脉曲张在平淡的体格检查期间被检测,并且预言与精索静脉曲张的青春期的介绍哪个将最终在青春期或成人生活显示出减少的阴囊的功能是困难的。作为在成年人,为在青少年的精索静脉曲张的处理的支柱是外科的修正。然而,不同于成年 varicocelectomy (microsurgical 途径是最普通) ,为青春期的精索静脉曲张的处理更经常是 laparoscopic。不过,治疗的目标是在青春期、成年的病人的一样。至于病人,并且什么类型治疗,,对待开始治疗是最好的争吵遗体。这评论将介绍青春期的精索静脉曲张的病原学,诊断和治疗的当前的理解。 | Koji Chiba Ranjith Ramasamy Dolores J Lamb Larry I Lipshultz | 2016 | Asian Journal of Andrology2016，18，2： | 14 |
| 6 | Hepatitis B and inflammatory bowel disease: Role of antiviral prophylaxis显示文摘Hepatitis B virus (HBV) is a very common infection worldwide. Its reactivation in patients receiving immunosuppression has been widely described as being associated with significant morbidity and mortality unless anti-viral prophylaxis is administered. Treatment in inflammatory bowel disease (IBD) patients has changed in recent years and immunosuppression and biological therapies are now used more frequently than before. Although current studies have reported an incidence of hepatitis B in inflammatory bowel disease patients similar to that in the general population, associated liver damage remains an important concern in this setting. Liver dysfunction may manifest in several ways, from a subtle change in serum aminotransferase levels to fulminant liver failure and death. Patients undergoing double immunosuppression are at a higher risk, and reactivation usually occurs after more than one year of treatment. As preventive measures, all IBD patients should be screened for HBV markers at diagnosis and those who are positive for the hepatitis B surface antigen should receive antiviral prophylaxis before undergoing immunosuppression in order to avoid HBV reactivation. Tenofovir/entecavir are preferred to lamivudine as nucleos(t)ide analogues due to their better resistance profile. In patients with occult or resolved HBV, viral reactivation does not appear to be a relevant issue and regular DNA determination is recommended during immunosuppression therapy. Consensus guidelines on this topic have been published in recent years. The prevention and management of HBV infection in IBD patients is addressed in this review in order to address practical | Pilar López-Serrano Jose Lázaro Pérez-Calle Maria Dolores Sánchez-Tembleque | 2013 | World Journal of Gastroenterology2013，19，9： | 12 |
| 7 | Efficacy of neoadjuvant therapy and surgical rescue for locally advanced hepatoblastomas:10 year single-center experience and literature review显示文摘AIM:To report our experience with long-term outcomes after multimodal management therapy.METHODS:An observational retrospective study was performed containing seven patients with hepatoblastoma(Hbl)treated in our institution,a tertiary referral center,from 2003 to 2011.Demographic,preoperative,surgical,and outcome variables were collected.A survival analysis and a review of the current literature related to combination neoadjuvant chemotherapy and surgical resection on Hbl were performed.RESULTS:The median age at surgery was 14.4 mo,with a male to female ratio of 4:3.Pretext staging at diagnosis was as follows:stageⅠ,4 cases;stageⅡ,2 patients;and stageⅢ,1 case.Mean pretreatment tumor volume was 735 cm3.Five out of seven patients received neoadjuvant chemotherapy according to SIOPEL-3 or SIOPEL-6 protocols.Tumor volume and alphafetoprotein levels significantly dropped after neoadjuvant therapy.Surgical procedures performed included hemihepatectomies,segmentectomies and atypical resection.All patients received chemotherapy after surgery.Median postoperative hospital stay was 8 d.All patients were alive and disease-free after a median follow-up period of 23 mo.With regards to the literature review,seventeen articles were found that were related to our search.CONCLUSION:Our series shows how multimodal management of Hbl,exhaustive control and a meticulous surgical approach leads to almost 100%complete resection with optimal postoperative results. | Dolores Ayllon Teran Oscar Gómez Beltran Rubén Ciria Bru Elena Mateos González María José Pea Rosa Antonio Luque Molina Pedro López Cillero Javier Briceo Delgado | 2014 | World Journal of Gastroenterology2014，20，29： | 12 |
| 8 | 高龄对男性生育力的影响显示文摘在过去的几十年里,现代社会的压力和期望使得育龄夫妇生育年龄总体推迟。已知女性在临近40岁时卵母细胞的产生将会明显减少,而高龄对男性精子生成能力的影响还知之甚少。男性生育年龄无明确限制,因而高龄对男性生育能力的影响还不完全清楚。本文将就目前已知的高龄对男性精子质量、生育能力和子代健康的影响进行综述,希望能在医生就男性推迟生育及高龄相关的风险咨询时起到一定的指导作用。 | Jason R Kovac Josephine Addai Ryan P Smith Robert M Coward Dolores J Lamb Larry I Lipshultz | 2013 | Asian Journal of Andrology2013，15，6： | 12 |
| 9 | High-throughput Phenotyping and Genomic Selection:The Frontiers of Crop Breeding Converge显示文摘Genomic selection (GS) and high-throughput phenotyping have recently been captivating the interest of the crop breeding com-munity from both the public and private sectors world-wide.Both approaches promise to revolutionize the prediction of complex traits,including growth,yield and adaptation to stress.Whereas high-throughput phenotyping may help to improve understanding of crop physiology,most powerful techniques for high-throughput field phenotyping are empirical rather than analytical and compa-rable to genomic selection.Despite the fact that the two method-ological approaches represent the extremes of what is understood as the breeding process (phenotype versus genome),they both consider the targeted traits (e.g.grain yield,growth,phenology,plant adaptation to stress) as a black box instead of dissecting them as a set of secondary traits (i.e.physiological) putatively related to the target trait.Both GS and high-throughput phenotyping have in common their empirical approach enabling breeders to use genome profile or phenotype without understanding the underlying biology.This short review discusses the main aspects of both approaches and focuses on the case of genomic selection of maize flowering traits and near-infrared spectroscopy (NIRS) and plant spectral reflectance as high-throughput field phenotyping methods for complex traits such as crop growth and yield. | Lloren Cabrera-Bosquet Jos Crossa Jarislav von Zitzewitz María Dolors Serret Jos Luis Araus | 2012 | Journal of Integrative Plant Biology2012，54，5： | 11 |
| 10 | Contribution of the ear and the flag leaf to grainfilling in durum wheat inferred from the carbon isotope signature: Genotypic and growing conditions effects显示文摘The ear, together with the flag leaf, is believed to play a major role as a source of assimilates during grain filling in C3cereals. However, the intrusive nature of most of the available methodologies prevents reaching conclusive results in this regard. This study compares the carbon isotope composition(d13C) in its natural abundance in the water‐soluble fractions of the flag leaf blade and the ear with the d13C of mature kernels to assess the relative contribution of both organs to grain filling in durum wheat(Triticum turgidum L. var.durum). The relative contribution of the ear was higher in landraces compared to modern cultivars, as well as in response to nitrogen fertilization and water stress. Such genotypic and environmentally driven differences were associated with changes in harvest index(HI), with the relative contribution of the ear being negatively associated with HI. In the case of the genotypic differences, the lower relative contribution of the ear in modern cultivars compared with landraces is probably associated with the appearance in the former of a certain amount of source limitation driven by a higher HI. In fact, the relative contribution of the ear was far more responsive to changes in HI in modern cultivars compared with landraces. | Rut Sanchez-Bragado Abdelhalim Elazab Bangwei Zhou Maria Dolors Serret Jordi Bort Maria Teresa Nieto-Taladriz José Luis Araus | 2014 | Journal of Integrative Plant Biology2014，56，5： | 10 |
| 11 | Wheat ear carbon assimilation and nitrogen remobilization contribute significantly to grain yield显示文摘The role of wheat ears as a source of nitrogen(N)and carbon(C) in the grain filling process has barely been studied. To resolve this question, five wheat genotypes were labeled with15N-enriched nutrient solution. N remobilization and absorption were estimated via the nitrogen isotope composition of total organic matter and Rubisco. Gas exchange analyses showed that ear photosynthesis contributed substantially to grain filling in spite of the great loss of C due to respiration. Of the total kernel N, 64.7% was derived from the N acquired between sowing and anthesis, while the remaining35.3% was derived from the N acquired between anthesis and maturity. In addition, 1.87 times more N was remobilized to the developing kernel from the ear than from the flag leaf. The higher yielding genotypes showed an increased N remobilization to the kernel compared to the lower yielding genotypes. Inaddition, the higher yielding genotypes remobilized more N from the ears to the kernel than the lower yielding genotypes,while the lower yielding genotypes remobilized more N from the flag leaf to the kernel. Therefore, the ears contribute significantly toward fulfilling C and N demands during grain filling. | Bangwei Zhou Maria Dolores Serret Abdelhalim Elazab Jordi Bort Pie José Luis Araus Iker Aranjuelo álvaro Sanz-Sáez | 2016 | Journal of Integrative Plant Biology2016，58，11： | 9 |
| 12 | Bone alterations in inflammatory bowel diseases显示文摘Inflammatory bowel diseases(IBDs)are characterized by a multifactorial partially unknown etiology that involves genetic,immunological and environmental factors.Up to 50%of IBD patients experience at least one extraintestinal manifestation;among them is the involvement of bone density which is referred to as metabolic bone disease(MBD),including osteopenia and osteoporosis.Bone alterations in IBDs population appear to have a multifactorial etiology:Decreased physical activity,inflammation-related bone resorption,multiple intestinal resections,dietary malabsorption of minerals and vitamin D deficiency,genetic factors,gut-bone immune signaling interaction,steroid treatment,microbiota and pathogenic micro-organisms interaction,and dietary malabsorption of minerals,that,all together or individually,may contribute to the alteration of bone mineral density.This review aims to summarize the prevalence and pathophysiology of metabolic bone alterations in IBD subjects outlining the main risk factors of bone fragility.We also want to underline the role of the screening and prophylaxis of bone alterations in Crohn’s disease and ulcerative colitis patients and the importance of treating appropriately MBD. | Dolores Sgambato Francesca Gimigliano Cristiana De Musis Antimo Moretti Giuseppe Toro Emanuele Ferrante Agnese Miranda Domenico De Mauro Lorenzo Romano Giovanni Iolascon Marco Romano | 2019 | World Journal of Clinical Cases2019，7，15： | 8 |
| 13 | Recurrent anal fistulae:Limited surgery supported by stem cells显示文摘AIM:To study the results of stem-cell therapy under a Compassionate-use Program for patients with recurrent anal fistulae.METHODS:Under controlled circumstances,and approved by European and Spanish laws,a Compassionate-use Program allows the use of stem-cell therapy for patients with very complex anal fistulae.Candidates had previously undergone multiple surgical interventions that had failed to resolve the fistulae,and presented symptomatic recurrence.The intervention consisted of limited surgery(with closure of the internal opening),followed by local implant of stem cells in the fistula-tract wall.Autologous expanded adipose-derived stem cells were the main cell type selected for implant.The first evaluation was performed on the 8th postoperative week;outcome was classified as response or partial response.Evaluation one year after the intervention confirmed if complete healing of the fistula was achieved.RESULTS:Ten patients(8 male)with highly recurrent and complex fistulae were treated(mean age:49years,range:28-76 years).Seven cases were nonCrohn’s fistulae,and three were Crohn’s-associated fistulae.Previous surgical attempts ranged from 3to 12.Two patients presented with preoperative incontinence(Wexner scores of 12 and 13 points).After the intervention,six patients showed clinical response on the 8th postoperative week,with a complete cessation of suppuration from the fistula.Three patients presented a partial response,with an evident decrease in suppuration.A year later,six patients(60%)remained healed,with complete reepithelization of the external opening.Postoperative Wexner Scores were 0 in six cases.The two patients with previous incontinence improved their scores from12 to 8 points and from 13 to 5 points.No adverse reactions or complications related to stem-cell therapy were reported during the study period.CONCLUSION:Stem cells are safe and useful for treating anal fistulae.Healing can be achieved in severe cases,sparing fecal incontinence risk,and improving previous scoring. | Damian Garcia-Olmo Hector Guadalajara Ines Rubio-Perez Maria Dolores Herreros Paloma de-la-Quintana Mariano Garcia-Arranz | 2015 | World Journal of Gastroenterology2015，21，11： | 7 |
| 14 | Conventional digital cameras as a tool for assessing leaf area index and biomass for cereal breeding显示文摘Affordable and easy-to-use methods for assessing biomass and leaf area index(LAI) would be of interest in most breeding programs. Here, we describe the evaluation of a protocol for photographic sampling and image analysis aimed at providing low-labor yet robust indicators of biomass and LAI. In this trial, two genotypes of triticale, two of bread wheat, and four of tritordeum were studied. At six dates during the growing cycle, biomass and LAI were measured destructively, and digital photography was taken on the same dates. Several vegetation indices were calculated from each image. The results showed that repeatable and consistent values of the indices were obtained in consecutive photographic samplings on the same plots. The photographic indices were highly correlated with the destructive measurements, though the magnitude of the correlation was lower after anthesis. This work shows that photographic assess-ment of biomass and LAI can be fast, affordable, have good repeatability, and can be used under bright and overcast skies. A practical vegetation index derived from pictures is the fraction of green pixels over the total pixels of the image, and as it shows good correlations with all biomass variables, is the most robust to lighting conditions and has easy interpretation. | Jaume Casadesús Dolors Villegas | 2014 | Journal of Integrative Plant Biology2014，56，1： | 7 |
| 15 | Men with a complete absence of normal sperm morphology exhibit high rates of success without assisted reproduction显示文摘在里面结合不孕, 0% 正常形式(NF ) 的反常严格的形态学是一个标准很快继续到在 vitro 授精(IVF ) 。自从没有数据,当前存在,我们与 0% NF 为人调查了结果没有帮助繁殖技术(艺术) 的使用,决定繁殖成功。有 0% NF 的 24 个人的一个队被识别(2010-2013 ) 随机与 ≥ 与 27 选择了人;是的 4% NF 控制。耐心的图表与联系并且管理同意的一块机构的评论板(IRB ) 的人一起被考察查明结果的电话问询表。在 2.5 年的一中部的后续时间以后,有 0% NF 的 29.2% 人没为他们的第一怀孕要求艺术(控制 =55.6% , P ≤0.05 ) 。当所有怀孕一起被分析时,有 0% NF 的人完成了 75% 没要求 IVF 的二十怀孕(控制 = 三十怀孕;76.7% 没要求 IVF ) 。人和女搭挡的平均年龄在有 0% NF 和 ≥ 的人之间是类似的; 4% NF。所有人有正常刺激滤泡的荷尔蒙(FSH ) ,睾丸激素,催乳激素,性别荷尔蒙绑定血球素(SHBG ) ,和 estradiol。尽管,当第一怀孕是一个自然概念(NC ) 时,全球精液参数在有 0% NF 的人是更坏的,有 0% NF (n = 7/7 ) 的人的 100% 并且 37.5% 控制(n = 3/8 ) 继续了经由 NC 有随后的怀孕。没有 IVF,有 0% NF 的人与 55.6% 控制相比在 29.2% 盒子中怀孕了。严格的形态学不应该被用来预言授精,怀孕,或实时出生潜力。在有 0% NF 的人,其他的形式应该被认为 IVF 以前立即。 | Jason R Kovac Ryan P Smith Miguel Cajipe Dolores J Lamb Larry I Lipshultz | 2017 | Asian Journal of Andrology2017，19，1： | 7 |
| 16 | Physiological traits contributed to the recent increase in yield potential of winter wheat from Henan Province, China显示文摘This experiment aims to test the traits responsible for the increase in yield potential of winter wheat released in Henan Province, China. Seven established cultivars released in the last 20 years and three advanced lines were assayed. The results showed that grain yield was positively correlated with harvest index(HI), kernel number per square meter, and aboveground biomass. In addition, the HI and aboveground biomass showed an increasing trend with the year of release.Therefore, we can conclude that bread wheat breeding advances during recent decades in Henan Province, China,have been achieved through an increase in HI, kernel number per square meter, and aboveground biomass. A higher d13C seems also to be involved in these advances, which suggests a progressive improvement in constitutive water use efficiency not associated with a trend towards lower stomatal conductance in the most recent genotypes. However, genetic advance Researchdoes not appear related to changes in photosynthesis rates on area basis when measured in the flag leaf or the spike,but only to a higher, whole‐spike photosynthesis. Results also indirectly support the concept that under potential yield conditions, the spike contributed more than the flag leaf to kernel formation. | Bangwei Zhou álvaro Sanz-Sáez Abdelhalim Elazab Tianmin Shen Rut Sánchez-Bragado Jordi Bort Maria Dolors Serret José Luis Araus | 2014 | Journal of Integrative Plant Biology2014，56，5： | 6 |
| 17 | Regulatory T cells in patients with inflammatory bowel diseases treated with adacolumn granulocytapheresis显示文摘AIM: To investigate if the clinical efficacy of granulocytes and monocytes by adsorption (GMA) is associated with an increased frequency of peripheral regulatory T cells (Tregs), as these cells have proven to be successful in suppressing inflammatory bowel disease (IBD) in animal models. METHODS: We report four cases of corticosteroid- dependent ulcerative colitis (UC) and two Crohn’s disease (CD) cases with severe cutaneous lesions who received GMA therapy. The frequency of CD4+ CD25high (Tregs) in peripheral blood was analyzed by flow cytometry and the expression of FoxP3 and TGF beta in purified CD4+ T cells was determined by real time PCR prior to and one month after the last apheresis session, and at the time of endoscopic and clinical assessing. RESULTS: Increased expression of Fox P3 mRNA was found in all five patients who responded to cytapheresis with remission of clinical symptoms, mucosal inflammation and cutaneous lesions, and an increased frequency of circulating Tregs was found in four patients. These changes were not observed in the patient with UC who did no respond to GMA. Variations in TGF-β (mRNA) did not parallel that of FoxP3 mRNA. CONCLUSION: The clinical efficacy of GMA on IBD and related extra intestinal manifestations was associated with an expansion of circulating CD4+ CD25+ Tregs and higher expression of FoxP3 in CD4+ T cells. Accordingly, an elevated CD4+ CD25+ FoxP3 may be a valuable index of remission in patients with IBD and other chronic relapsing-remitting inflammatory conditions during treatment with GMA. | Emilio Cuadrado Marta Alonso Maria Dolores de Juan Pilar Echaniz Juan Ignacio Arenas | 2008 | World Journal of Gastroenterology2008，14，10： | 6 |
| 18 | Further the liquid biopsy:Gathering pieces of the puzzle of genometastasis theory显示文摘Metastasis is the major cause of mortality in cancer disease and still constitutes one of the most controversial mechanism,not yet fully understood.What is almost beyond doubt is that circulatory system is crucial for cancer propagation.Regarding this system,much attention has been recently paid to liquid biopsy.This technique is aimed to detect circulating tumor cells (CTCs) and circulating nucleic acids so it can be used as a tool for diagnostic,prognostic and follow-up of patients.Whereas CTCs tend to be scarce in serum and plasma from cancer patient,abundant circulating nucleic acids can be detected in the same location.This fact,together with the genetic origin of cancer,stands out the relevance of circulating nucleic acids and shed light into the role of nucleic acids as drivers of metastasis,a recently discovered phenomenon called Genometastasis.This innovative theory supports the transfer of oncogenes from cancer cells to normal and susceptible cells located in distant target organs through circulatory system.What is more,many biological processes haven been described to deliver and secrete circulating nucleic acids into the circulation which can allow such horizontal transfer of oncogenes.In this review,we focus not only on these mechanisms but also we demonstrate its putative role in cancer propagation and give insights about possible therapeutic strategies based on this theory.Our objective is to demonstrate how findings about cell-to-cell communications and previous results can agree with this unprecedented theory. | Ana García-Casas Dolores C García-Olmo Damián García-Olmo | 2017 | World Journal of Clinical Oncology2017，8，5： | 5 |
| 19 | 黄连素及其与BCNU联合应用治疗恶性脑肿瘤的实验研究显示文摘本文对黄连素的抗脑肿瘤作用,以及与1,3-bis(2-chloroethyl)-l-nitrosourea(BC-NU)联合应用进行了实验研究。用集落生成率测定(CFE)对6个人类脑胶质瘤细胞株和大白鼠9L脑肿瘤细胞(胶质肉瘤)进行了离体研究。用姐妹染色体交换法(SCE),对9L脑肿瘤细胞进行了评价。同时,在活体实验中用集落生成率测定对脑内固体9L脑肿瘤的治疗进行了分析。在BCNU的浓度为23μm单独使用时,对6个人类脑胶质瘤细胞株可获得平均为43%的细胞杀灭作用(0.24Log Kill)。而在黄连素与23μmBCNV联合应用治疗中,则可见到平均为97%的细胞杀灭相加作用(1.55Log Kill)。在采用150μg/ml黄连素离体治疗中,9L细胞姐妹染色体相对数的增高超过底数的2.7倍。在活体实验中,黄连素与BCNU联合应用也显示出细胞毒性相加作用。 | 张荣勋 MarkL.Posenblum Dolores V.Dougherty | 1993 | 中国神经精神疾病杂志1993，19，2： | 5 |
| 20 | In drug-induced, immune-mediated hepatitis, interleukin-33 reduces hepatitis and improves survival independently and as a consequence of FoxP3+ T-cell activity显示文摘Immune-mediated,drug-induced hepatitis is a rare complication of halogenated volatile anesthetic administration.IL-4-regulated Th2-polarized reactions initiate this type and other types of hepatitis,while the mechanisms that regulate the severity remain elusive.IL-33 is an innate,IL-4-inducing,Th2-polarizing cytokine that has been detected in patients with liver failure and has been associated with upregulated ST2+Foxp3+CD4+CD25+T cells;however,roles for IL-33 in drug-induced hepatitis are unclear.We investigated IL-33 in an anesthetic,immune-mediated hepatitis modeled in BALB/c,IL-33−/−and ST2−/−mice,as well as in patients with anesthetic hepatitis.The hepatic IL-33 and ST2 levels were elevated in BALB/c mice(p<0.05)with hepatitis,and anti-IL-33 diminished hepatitis(p<0.05)without reducing IL-33 levels.The complete absence of IL-33 reduced IL-10(p<0.05)and ST2+Foxp3+CD4+CD25+T cells(p<0.05),as well as reduced the overall survival(p<0.05),suggesting suppressive roles for IL-33 in anesthetic,immune-mediated hepatitis.All of the mice demonstrated similar levels of CD4+T-cell proliferation following direct Tcell receptor stimulation,but we detected splenic IL-33 and ST2-negative Foxp3+CD4+CD25+T cells in ST2−/−mice that developed less hepatitis than BALB/c mice(p<0.05),suggesting that ST2-negative Foxp3+CD4+CD25+T cells reduced hepatitis.In patients,serum IL-33 and IPEX levels were correlated in controls(r2=0.5,p<0.05),similar to the levels in mice,but not in anesthetic hepatitis patients(r2=0.01),who had elevated IL-33(p<0.001)and decreased IPEX(p<0.01).Our results suggest that,in anesthetic,immune-mediated hepatitis,IL-33 does not regulate the CD4+T-cell proliferation that initiates hepatitis,but IL-33,likely independent of ST2,reduces hepatitis via upregulation of Foxp3+CD4+CD25+T cells.Further studies are needed to translate the role of IL-33 to human liver disease. | Merylin Cottagiri Maeva Nyandjo Matthew Stephens Joel J.Mantilla Hirohisa Saito Ian R.Mackay Noel R.Rose Dolores B.Njoku | 2019 | Cellular & Molecular Immunology2019，16，8： | 4 |
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