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| 1 | No evidence of HPV DNA in esophageal squamous cell carcinoma in a population of Southern Brazil显示文摘AIM:To investigate the association between human papillomavirus(HPV)and esophageal squamous cell carcinoma(ESCC)in southern Brazil.METHODS:We studied 189 esophageal samples from125 patients from three different groups:(1)102 biopsies from 51 patients with ESCC,with one sample from the tumor and another from normal esophageal mucosa distant from the tumor;(2)50 esophageal biopsies from 37 patients with a previous diagnosis of head and neck squamous cell carcinoma(HNSCC);and(3)37 biopsies from esophageal mucosa with normal appearance from 37 dyspeptic patients,not exposed to smoking or alcohol consumption.Nested-polymerase chain reaction(PCR)with the MY09/11 and GP5/6 L1primers was used to detect HPV L1 in samples fixed in formalin and stored in paraffin blocks.All PCR reactions were performed with a positive control(cervicovaginal samples),with a negative control(Human Genomic DNA)and with a blank reaction containing all reagents except DNA.We took extreme care to prevent DNA contamination in sample collection,processing,and testing.RESULTS:The histological biopsies confirmed the diagnosis of ESCC in 52 samples(51 from ESCC group and 1 from the HNSCC group)and classified as well differentiated(12/52,23.1%),moderately differentiated(27/52,51.9%)or poorly differentiated(7/52,13.5%).One hundred twenty-eight esophageal biopsies were considered normal(51 from the ESCC group,42 from the HNSCC group and 35 from dyspeptic patients).Nine had esophagitis(7 from the HNSCC and 2 from dyspeptic patients).Of a total of 189 samples,only 6 samples had insufficient material for PCR analysis:1 from mucosa distant from the tumor in a patient with ESCC,3from patients with HNSCC and 2 from patients without cancer.In 183 samples(96.8%)GAPDH,G3PDH and/orβ-globin were amplified,thus indicating the adequacy of the DNA in those samples.HPV DNA was negative in all the 183 samples tested:52 with ESCC,9 with esophagitis and 122 with normal esophageal mucosa.CONCLUSION:There was no evidence of HPV infection in different ESCC from southern Brazil. | Luís Carlos Moreira Antunes Joo Carlos Prolla Antonio de Barros Lopes Marta Pires da Rocha Renato Borges Fagundes | 2013 | World Journal of Gastroenterology2013，19，39： | 4 |
| 2 | Chronic myeloid leukemia-from the Philadelphia chromosome to specific target drugs:A literature review显示文摘Chronic myeloid leukemia(CML)is a myeloproliferative neoplasm and was the first neoplastic disease associated with a well-defined genotypic anomaly―the presence of the Philadelphia chromosome.The advances in cytogenetic and molecular assays are of great importance to the diagnosis,prognosis,treatment,and monitoring of CML.The discovery of the breakpoint cluster region(BCR)-Abelson murine leukemia(ABL)1 fusion oncogene has revolutionized the treatment of CML patients by allowing the development of targeted drugs that inhibit the tyrosine kinase activity of the BCR-ABL oncoprotein.Tyrosine kinase inhibitors(known as TKIs)are the standard therapy for CML and greatly increase the survival rates,despite adverse effects and the odds of residual disease after discontinuation of treatment.As therapeutic alternatives,the subsequent TKIs lead to faster and deeper molecular remissions;however,with the emergence of resistance to these drugs,immunotherapy appears as an alternative,which may have a cure potential in these patients.Against this background,this article aims at providing an overview on CML clinical management and a summary on the main targeted drugs available in that context. | Mariana Miranda Sampaio Maria Luísa Cordeiro Santos Hanna Santos Marques Vinícius Lima de Souza Gonçalves Glauber Rocha Lima Araújo Luana Weber Lopes Jonathan Santos Apolonio Camilo Santana Silva Luana Kauany de SáSantos Beatriz Rocha Cuzzuol Quézia Estéfani Silva Guimarães Mariana Novaes Santos Breno Bittencourt de Brito Filipe Antônio França da Silva Márcio Vasconcelos Oliveira Cláudio Lima Souza Fabrício Freire de Melo | 2021 | World Journal of Clinical Oncology2021，12，2： | 3 |
| 3 | Physiological Effect of Kinetin on the Photosynthetic Apparatus and Antioxidant Enzymes Activities During Production of Anthurium显示文摘The results observed in the literature raise the hypothesis according to which cytokinin plays important roles in photosynthetic metabolisms and antioxidant enzymes. Thus, the study aimed to evaluate the effect of foliar application of the isolated cytokinin kinetin at the production cycle, seeking to analyze its effects on enzyme activity and photosynthetic parameters. The plants treated with CK presented reduction of leaf CO_2 assimilation rate(Pn) and stomatal conductance(Gs), while that transpiration rate(Tr) was unaffected. The internal CO_2 concentrations decreased with the increase in cytokinin levels, but were maintained under CK 50 mg·L^(-1). The plants treated with CK 75 mg·L^(-1) was verified higher carboxylation efficiency(Pn/Ci), which was associated to values of CO_2 assimilation and transpiration unaltered. Apparent electron transport rate showed variations in the concentration of 25 mg·L^(-1). Considering the study of enzyme activity, on the other hand, it cannot be stated that kinetin has an effective action in delaying oxidative damage. It presents mixed results, since an efficiency in the application of cytokinin was not observed, presenting induction levels of ascorbate peroxidase activity. Thus, further research is needed to determine more precisely the effects of kinetin on gas exchange and antioxidant enzymes in anthurium plants. | de MOURA Fabiana B. VIEIRA Marcos R. da S. SIMOES Adriano do N. FERREIRA-SILVA Sérgio L. de SOUZA Carlos A.V. de SOUZA Eduardo S. da ROCHA Alexandre T. da SILVA Luzia F. JUNIOR Miguel A. | 2018 | Horticultural Plant Journal2018，4，5： | 3 |
| 4 | Sex-specific effects of Eugenia punicifolia extract on gastric ulcer healing in rats显示文摘AIM To evaluate the sex-specific effects of a hydroalcoholic extract from Eugenia punicifolia(HEEP) leaves on gastric ulcer healing.METHODS In this rat study involving males, intact(cycling) females, and ovariectomized females, gastric ulcers were induced using acetic acid. A vehicle, lansoprazole, or HEEP was administered for 14 d after ulcer induction. Body weight was monitored throughout the treatment period. At the end of treatment, the rats were euthanized and the following in vivo and in vitro investigations were performed: macroscopic examination of the lesion area and organ weights, biochemical analysis, zymography, and evaluation of protein expression levels. Additionally, the concentration-dependent effect of HEEP was evaluated in terms of subacute toxicity and cytotoxicity.RESULTS Compared to the vehicle, HEEP demonstrated a great healing capacity by substantially reducing the ulcerative lesion area in males(52.44%), intact females(85.22%), and ovariectomized females(65.47%), confirming that HEEP accelerates the healing of acetic acidinduced gastric lesions and suggesting that this effect is modulated by female sex hormones. The antiulcer effect of HEEP was mediated by prostaglandin E2 only in male rats. Overall, the beneficial effect of HEEP was the highest in intact females. Notably, HEEP promoted the expression of vascular endothelial growth factor(intact vs ovariectomized females) and decreased the expression of Caspase-8 and Bcl-2(intact female vs male or ovariectomized female). Additionally, HEEP enhanced fibroblast proliferation and migration into a wounded area in vitro, confirming its healing effect. Finally, no sign of subacute toxicity or cytotoxicity of HEEP was observed.CONCLUSION In gastric ulcers, HEEP-induced healing(modulated by female sex hormones; in males, mediated by prostaglandin) involves extracellular matrix remodeling, with gastric mucosa cell proliferation and migration. | Larissa Lucena Périco Vinícius Peixoto Rodrigues Rie Ohara Gabriela Bueno Vania Vasti Alfieri Nunes Raquel Cássia dos Santos Ana Carolina Lima Camargo Luis Antuio Justulin Joior Sérgio Faloni de Andrade Viviane Miranda Bispo Steimbach Luísa Mota da Silva Lúcia Regina Machado da Rocha Wagner Vilegas Catarina dos Santos Clélia Akiko Hiruma-Lima | 2018 | World Journal of Gastroenterology2018，24，38： | 3 |
| 5 | Probiotic potential of Mi-nas fresh cheese prepared with the addition of Lactobacillus paracasei显示文摘 | Buriti F A da Rocha J S Assis E G | | 0，，02： | 1 |
| 6 | Probiotic potential of Minas fresh cheese prepared with the addition of Lactobacillus paracasei 显示文摘 | BURITI F C A DA ROCHA J S ASSIS E G | 2005 | Lebensmittel-Wissenschaft und Tehonlogie2005，38，2： | 1 |
| 7 | Molecular differenc- es between hydrocarbon and fluorocarbon surfaetants at the COJ water interface 显示文摘 | Stone M T Da Rocha S R P Rossky P J | 2003 | Journal of Physics Chemistry2003，107，10： | 1 |
| 8 | Anticancer, antichemotactic and antimicrobial activities of marine sponges collected off the coast of Santa Catarina, southern Brazil显示文摘 | Noel R Monks Clea Lerner Amélia T Henriques Fabiane M Farias Elfrides E.S Schapoval Edna S Suyenaga Adriana B da Rocha Gilberto Schwartsmann Beatriz Mothes | 2002 | Journal of Experimental Marine Biology and Ecology2002，，1： | 1 |
| 9 | Water in car- bon dioxide macroemulsions and miniemulsions with a hydrocar- bon surfactant显示文摘 | Johnston K P Cho Dongman Da Rocha S R P | 2001 | Langmuir2001，17，23： | 1 |
| 10 | Widely linear adaptive equalization of digital communication channel 显示文摘 | de Aquino F J A Resende L S da Rocha C A F | 2010 | IEEE Latin America Transactions2010，8，1： | 1 |
| 11 | Inducible expres- sion of (V600E) Braf using tyrosinase-driven Cre recombinase results in embryonic lethality 显示文摘 | Dhomen N Da Rocha I)ias S Hayward R | 2010 | Pigment Cell Melanoma Res2010，23，1： | 1 |
| 12 | Two opposite signaling outputs are driven by the KIR2DL1 receptor in human CD4^+ T cells显示文摘 | Fourmentraux-Neves E Jalil A Da Rocha S | 2008 | Blood2008，112，6： | 1 |
| 13 | Surfactant-modified CO2-water interface: A molecular view显示文摘 | Da Rocha S R P Johnstom K P Rossky P J | 2002 | J Phys Chem B2002，106，13： | 1 |
| 14 | The decreased susceptibility of Bcr/Abl targets to NK cell-mediated lysis in response to imatinib mesylate involves modulation of NKG2D ligands, GM1 expression, and synapse formation 显示文摘 | Cebo C Da Rocha S Wittnebel S | 2006 | J Immunol2006，176，： | 1 |
| 15 | Genomic imprinting, growth control and the allocation of nutritional resources: conse- quences for postnatal life 显示文摘 | CHARALAMBOUS M DA ROCHA S T FERGU- SON-SMITH A C | 2007 | Current Opinion in En- docrinology Diabetes and Obesity2007，14，1： | 1 |
| 16 | A mass classification using spatial diversity approaches in mammography images for false positive reduction显示文摘 | Braz J G da Rocha S V Gattass M | 2013 | Expert Systems with Applications2013，40，18： | 1 |
| 17 | Marine organisms as a source of new anticancer agents 显示文摘 | Gilberto S da Rocha AB Robert GS | 2001 | Oncol2001，2，： | 1 |
| 18 | Genomic imprinting at the mammalian Dlk1-Dio3 domain显示文摘 | DA ROCHA S T EDWARDS C A ITO M | 2008 | Trends Genet2008，24，6： | 1 |
| 19 | Surfactant-modified CO2-water interface : a molecular view 显示文摘 | da Rocha S R P Johnston K P Rossky P J | 2002 | Journal of Physical Chemistry B2002，106，13： | 1 |
| 20 | Effects of carbon and nitrogen sources on the proteome of Pseudomonas aeruginosa PA1 during rhamnolipid production显示文摘 | REIS R S da ROCHA S L G CHAPEAUROUGE D A | 2010 | Process Biochem2010，45，9： | 1 |
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