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| 1 | Liver fat deposition and mitochondrial dysfunction in morbid obesity:An approach combining metabolomics with liver imaging and histology显示文摘AIM: To explore the usefulness of magnetic resonance imaging(MRI) and spectroscopy(MRS) for assessment of non-alcoholic fat liver disease(NAFLD) as compared with liver histological and metabolomics findings. METHODS: Patients undergoing bariatric surgery following procedures involved in laparoscopic sleeve gastrectomy were recruited as a model of obesityinduced NAFLD in an observational, prospective, singlesite, cross-sectional study with a pre-set duration of 1 year. Relevant data were obtained prospectively and surrogates for inflammation, oxidative stress and lipid and glucose metabolism were obtained through standard laboratory measurements. To provide reliable data from MRI and MRS, novel procedures were designed to limit sampling variability and other sources of error using a 1.5T Signa HDx scanner and protocols acquired from the 3D or 2D Fat SAT FIESTA prescription manager. We used our previously described 1H NMRbased metabolomics assays. Data were obtained immediately before surgery and after a 12-mo period including histology of the liver and measurement of metabolites. Values from 1H NMR spectra obtained after surgery were omitted due to technical limitations.RESULTS: MRI data showed excellent correlation with the concentration of liver triglycerides, other hepatic lipid components and the histological assessment, w h i c h e xc l u d e d t h e p r e s e n c e o f n o n-a l c o h o l i c steatohepatitis(NASH). MRI was sufficient to follow up NAFLD in obese patients undergoing bariatric surgery and data suggest usefulness in other clinical situations. The information provided by MRS replicated that obtained by MRI using the-CH3 peak(0.9 ppm), the-CH2- peak(1.3 ppm, mostly triglyceride) and the-CH=CH- peak(2.2 ppm). No patient depicted NASH. After surgery all patients significantly decreased their body weight and steatosis was virtually absent even in patients with previous severe disease. Improvement was also observed in the serum concentrations of selected variables. The most relevant findings using metabolomics indicate increased levels of triglyceride and monounsaturated fatty acids in severe steatosis but those results were accompanied by a significant depletion of diglycerides, polyunsaturated fatty acids, glucose-6-phosphate and the ATP/AMP ratio. Combined data indicated the coordinated action on mitochondrial fat oxidation and glucose transport activity and may support the consideration of NAFLD as a likely mitochondrial disease. This concept may helpto explain the dissociation between excess lipid storage in adipose tissue and NAFLD and may direct the search for plasma biomarkers and novel therapeutic strategies. A limitation of our study is that data were obtained in a relatively low number of patients.CONCLUSION: MRI is sufficient to stage NAFLD in obese patients and to assess the improvement after bariatric surgery. Other data were superfluous for this purpose. | Nahum Calvo Raúl Beltrán-Debón Esther Rodríguez-Gallego Anna Hernández-Aguilera Maria Guirro Roger Mariné-Casadó Lidón Millá Josep M Alegret Fàtima Sabench Daniel del Castillo María Vinaixa Miguelàngel Rodríguez Xavier Correig Roberto García-álvarez Javier A Menendez Jordi Camps Jorge Joven | 2015 | World Journal of Gastroenterology2015，21，24： | 2 |
| 2 | Decontamination of beef carcass surface tissue by steam vacuuming alone and combined wit h hot water and Iactic acid sprays显示文摘 | Castillo A L ucia L M Goodson K J | 1999 | J Food Prot1999，62，： | 1 |
| 3 | An Assessment of Ad- vanced Mobile Services Acceptance: Contributions from TAM and Diffusion Theory Models 显示文摘 | Nicolas C L Castillo F J M Bouwman H | 2008 | Information & Management2008，45，6： | 1 |
| 4 | Clinical applications of proton MR spectroscopy显示文摘 | Castillo M Kwock L Mukherji SK | 1996 | AJNR1996，17，1： | 1 |
| 5 | Apparent diffusion coefficients in the evaluation of high-grade cerebral gliomas显示文摘 | Castillo M Smith JK Kwock L | 2001 | AJNR Am J Neuroradiol2001，22，1： | 1 |
| 6 | Solid-phase microcxtraction as a methodology in the identification of irradiated ground beef显示文摘 | Caja M M Castillo M L R Blanch G P | 2008 | Food Chemistry2008，110，2： | 1 |
| 7 | Apparent diffusion coefficients in the evaluation of high-grade cerebral gliomas显示文摘 | Castillo M Smith JK Kwock L | 2001 | AJNR2001，22，1： | 1 |
| 8 | Postirradiation polymerization of vinyl monomers on poly(ethylene terephthalate) 显示文摘 | HSIEH Y L SHINAWATRA M CASTILLO M D | 1986 | Journal of Applied Polymer Science1986，31，2： | 1 |
| 9 | In-plant evaluation of a lactic acid treatment for reduction of bacteria on chilled beef carcasses显示文摘 | CASTILLO A LUCIA L M MERCADO I | 2001 | Food Protection2001，，64： | 1 |
| 10 | Isolated soy protein improves endothelial function in postmenopa- usal hypercholesterolemic women 显示文摘 | Cuevas A M Irribarra V L Castillo 0 A | 2003 | European Journal of Clinical Nutrition2003，57，8： | 1 |
| 11 | Study of solid contamination in ball bearings through vibration and wear analyses显示文摘 | M M Maru R S Castillo L R Padovese | 2007 | Tribology International2007，40，3： | 1 |
| 12 | Polishing of beryllium capsules to meet N1F specifications显示文摘 | Hoope M L Castillo E | 2006 | Journal de Plysique2006，4，89： | 1 |
| 13 | Enantiomeric purity of ( +/- )-Methyl Jasmonate in fresh leaf samples and commercial fragrances 显示文摘 | Castillo M L R D Blanch F P | 2007 | Journal of Separation Sci- ence2007，30，13： | 1 |
| 14 | Physicochem~cal, structural, and digesti~ihty properties of enzymatic modified plantain and mango starches 显示文摘 | Casarrubias- Castillo M G Hamaker B R Rodriguez-Ambriz S L Be~o-P~rez L A_ | 2012 | Starch/Starke2012，64，4： | 1 |
| 15 | Clinical application of proton spectroscopy显示文摘 | Castillo M Kwork L Mukherji SK | 1996 | Am J Neuroradiol1996，17，1： | 1 |
| 16 | Apparent diffusion coefficients in the evaluation of high-grade cerebral gliomas显示文摘 | Castillo M Smith KJ Kwock L | 2001 | AJNR2001，22，1： | 1 |
| 17 | Determination of differences in anatomical and chemical characteristics of tension and opposite wood of 8 years old Eucalyptus globulus 显示文摘 | Aguayo M G Quintupill L Castillo R | 2010 | Maderas Ciencia Technologiz2010，12，3： | 1 |
| 18 | Kinetic parameters for denaturation of bovine milk lactoferrin 显示文摘 | Sanehez L Peiro J M Castillo H | 1992 | Journal of Food Science1992，57，4： | 1 |
| 19 | Adenosquamous carcinoma of the head and neck: criteria for diagnosis in a study of 12 cases 显示文摘 | Alos L Castillo M Nadal A | 2004 | Histopathology2004，44，6： | 1 |
| 20 | Stabilization of All-trans-lycopene from Tomato by Encapsulation Using Cyclodextrins显示文摘 | BLANCH G P RUTZ DEL CASTILLO M L CAJA M M et at | 2007 | Food Chem2007，105，4： | 1 |
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