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| 1 | Daily genetic profiling indicates JAK/STAT signaling promotes early hepatic stellate cell transdifferentiation显示文摘AIM: To identify signaling pathways and genes that initiate and commit hepatic stellate cells (HSCs) to transdifferentiation. METHODS: Primary HSCs were isolated from male Sprague-Dawley rats and cultured on plastic for 0-10 d. Gene expression was assessed daily (quiescent to day 10 culture-activation) by real time polymerase chain reaction and data clustered using AMADA software. The significance of JAK/STAT signaling to HSC transdifferentiation was determined by treating cells with a JAK2 inhibitor. RESULTS: Genetic cluster analyses, based on expression of these 21 genes, showed similar expression profiles on days 1-3, days 5 and 6, and days 7-10, while freshly isolated cells (day Q) and day 4 cells were genotypically distinct from any of the other days. Additionally, gene expression clustering revealed strong upregulation of interleukin-6, JAK2 and STAT3 mRNA in the early stages of activation. Inhibition of the JAK/STAT signaling pathway impeded the morphological transdifferentiation of HSCs which correlated with decreased mRNA expression of several profibrotic genes including collagens, α-SMA, PDGFR and TGFβR. CONCLUSION: These data demonstrate unique clustered genetic profiles during the daily progression of HSC transdifferentiation and that JAK/STAT signaling may be critical in the early stages of transdifferentiation. | Ashley M Lakner Cathy C Moore Alyssa A Gulledge Laura W Schrum | 2010 | World Journal of Gastroenterology2010，16，40： | 23 |
| 2 | Acute pancreatitis in aging animals:Loss of pancreatitis-associated protein protection?显示文摘AIM:To investigate the effect of age on severity of acute pancreatitis(AP) using biochemical markers,histology and expression of the protective pancreatitisassociated proteins(PAPs).METHODS:AP was induced via intraductal injection of 4% sodium taurocholate in young and old rats.Sera and pancreata were assayed at 24 h for the parameters listed above;we also employed a novel molecular technique to assess bacterial infiltration using polymerase chain reaction to measure bacterial genomic ribosomal RNA.RESULTS:At 24 h after induction of AP,the pancreata of older animals had less edema(mean ± SE histologic score of young vs old:3.11 ± 0.16 vs 2.50 ±-0.11,P < 0.05),decreased local inflammatory response(histologic score of stromal infiltrate:3.11 ± 0.27 vs 2.00 ± 0.17,P < 0.05) and increased bacterial infiltration(174% ± 52% increase from sham vs 377% ± 4%,P < 0.05).A decreased expression of PAP1 and PAP2 was demonstrated by Western blotting analysis and immunohistochemical staining.There were no differences in serum amylase and lipase activity,or tissue myeloperoxidase or monocyte chemotactic protein-1 levels.However,in the most-aged group,serum C-reactive protein levels were higher(young vs old:0.249 ± 0.04 mg/dL vs 2.45 ± 0.68 mg/dL,P < 0.05).CONCLUSION:In older animals,there is depressed PAP expression related to a blunted inflammatory response in AP which is associated with worsened bacterial infiltration and higher C-reactive protein level;this may explain the more aggressive clinical course. | Sophia Fu Albert Stanek Cathy M Mueller Nefertti A Brown Chongmin Huan Martin H Bluth Michael E Zenilman | 2012 | World Journal of Gastroenterology2012，18，26： | 5 |
| 3 | Fmrl knockout mice : a model to study fragile X mental retardation显示文摘 | Cathy E Bakker A Coleta V | 1994 | Cell1994，78，： | 1 |
| 4 | Adenomatous polyposis coli - mediated control of β - catenin is essential for both chondrogenic and osteogenic differentiation of skeletal precursors 显示文摘 | Razvan LM Marcel K Cathy A J | 2009 | BMC Der Biol2009，9，： | 1 |
| 5 | Weight and symptom diary for self-monitoring in heart failure 显示文摘 | Cathy A Lucille Travis Tanya T | 2008 | J Cardiovasc Nurs2008，22，5： | 1 |
| 6 | Evidence-based systematic review:Effects of oral motor interventions on feeding and swallowing in preterm infants显示文摘 | Joan A Healther C Cathy L | 2010 | American Journal of Speech-Language Pathology2010，19，： | 1 |
| 7 | Permeability of shaly sands显示文摘 | Revil A Cathies L M | 1999 | Water Resources Research1999，35，3： | 1 |
| 8 | Outbreaks of renal failure associated with melamine and cyanuric acid in dogs and cats in 2004 and 2007 显示文摘 | Cathy A Brown Kyu Shik Jeong Robert H | 2007 | Journal of Veterinary Diagnostic Investigation2007，19，5： | 1 |
| 9 | Sweet com(Zea mays) hybrid tolerance to nicosulfuron显示文摘 | Cathy A Morton R Gordon Harvey | 1992 | Weed Technology1992，，： | 1 |
| 10 | Sweet corn(Zea mays) hybrid tolerance tonicosulfuron显示文摘 | Cathy A M Harvey R G | 1992 | Weed Technology1992，，： | 1 |
| 11 | The effetcs of oral-motor exercises on swallowing in children: an evidence-based systematic re- view显示文摘 | Joan A Heather C Cathy L | 2010 | Developmental Medicine & Child Neurology2010，52，： | 1 |
| 12 | Evaluation of transgenic tomato plants expressing an additional phytoene synthase in a fruit-specific manner显示文摘 | Paul D F Susanne R Cathie A S | 2002 | Proceedings of the National Academy of Sciences2002，99，2： | 1 |
| 13 | Extractive fermentation of aroma with supercritical C02 显示文摘 | Cathy E Fabre Condoret J S Marty A | 1999 | Biotechnology and Bioengineering1999，64，4： | 1 |
| 14 | Radial glial cell line C6-R integrates preferentially in adult white matter and facilitates migration of CO-implanted neurons in bibo显示文摘 | Homigo A Cathy Mc Nothial JM | 2001 | Exp Neurol2001，168，2： | 1 |
| 15 | Predicting Young Children's Externalizing Problems Interactions among Ef- fortful Control, Parenting, and Child Gender 显示文摘 | Annemiek Karreman Cathy van Tuijl Marcel A G | 2009 | Merrill-Pa Mer Quarterly2009，55，2： | 1 |
| 16 | Early use of supplemental parenteral nutrition in critically ill patients-Results of an international multicenter observational study显示文摘 | Jim K Cathy A Leah G | | 0，，12： | 1 |
| 17 | A chaotic heat - exchanger for PEMFC cooling applications 显示文摘 | YAHIA L BRUNO A CATHY C | 2005 | Journal of Power Sources2005，，156： | 1 |
| 18 | Toxicity of 1,4-dichlorobenzene in sediments to juvenile polychaete worms显示文摘 | McPherson Cathy A Tang Armando Chapman Peter M | 2002 | Marine Pollut Bulletin2002，44，： | 1 |
| 19 | Conquering pulmonary embolism显示文摘 | Cathy A | 2010 | OR Nurse2010，4，5： | 1 |
| 20 | A multicenter comparison of intraaortic balloon pump utilization in isolated coronary artery bypass graft surgery显示文摘 | Roger J.F Baskett Gerald T O’Connor Gregory M Hirsch William A Ghali Kathy Sabadosa Jeremy R Morton Cathy S Ross Felix Hernandez William C Nugent Stephen J Lahey Donato A Sisto Lawrence J Dacey John D Klemperer Robert E Helm Andrew Maitland | 2003 | The Annals of Thoracic Surgery2003，，6： | 1 |
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