|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Early dynamic transcriptomic changes during preoperative radiotherapy in patients with rectal cancer: A feasibility study显示文摘AIM: To develop novel biomarkers of rectal radiotherapy, we measured gene expression profiles on biopsies taken before and during preoperative radiotherapy. METHODS: Six patients presenting with a locally advanced rectal cancer (T>T2, N0/Nx, M0) eligible for preoperative radiotherapy (45 Gy in 25 fractions) were selected in a pilot study. Six tumor and 3 normal tissues biopsies were taken before and during radiotherapy,after a dose of 7.2 Gy at a median time of 1 h following irradiation (0:27-2:12). Tumor or normal tissue purity was assessed by a pathologist prior to RNA extraction. Mean RNA content was 23 μg/biopsy (14-37) before radiotherapy and 22.7 μg/biopsy (12-35) during radiotherapy. After RNA amplification, biopsies were analysed with 54K HG-U133A Plus 2.0 Affymetrix expression micro-arrays. Data were normalized according to MAS5 algorithm. A gene expression ratio was calculated as: (gene expression during radiotherapy-gene expression before radiotherapy)/gene expression before radiotherapy. Were selected genes that showed a ratio higher than ± 0.5 in all 6 patients. RESULTS: Microarray analysis showed that preoperative radiotherapy significantly up-regulated 31 genes and down-regulated 6 genes. According to the Gene Ontology project classification, these genes are involved in protein metabolism (ADAMDEC1 ; AKAP7 ; CAPN5 ; CLIC5 ; CPE ; CREB3L1 ; NEDD4L ; RAB27A), ion transport (AKAP7 ; ATP2A3 ; CCL28 ; CLIC5 ; F2RL2 ; NEDD4L ; SLC6A8), transcription (AKAP7 ; CREB3L1 ; ISX ; PAB-PC1L ; TXNIP), signal transduction (CAPN5 ; F2RL2 ; RA- B27A ; TNFRSF11A), cell adhesion (ADAMDEC1 ; PXDN ; SPON1 ; S100A2), immune response (CCL28 ; PXDN ; TNFRSF11A) and apoptosis (ITM2C ; PDCD4 ; PVT1). Up-regulation of 3 genes (CCL28 ; CLIC5 ; PDCD4) was detected by 2 different probes and up-regulation of 2 genes (RAB27A ; TXNIP) by 3 probes. CONCLUSION: Micro-arrays can efficiently assess early transcriptomic changes during preoperative radiotherapy for rectal cancer, and may help better understand tumor radioresistance. | Stephane Supiot Wilfried Gouraud Loc Campion Pascal Jezéquel Bruno Buecher Josiane Charrier Marie-Francoise Heymann Marc-Andre Mahé Emmanuel Rio Michel Chérel | 2013 | World Journal of Gastroenterology2013，19，21： | 2 |
| 2 | Siliclastic sequence stratigraphy in well logs, cores, and outcrop concepts for high-resolution correlation of time and facies显示文摘 | Van Wagoner J C Mitchum R M Campion K M | 1990 | AAPG Methods in Exploration Series1990，7，： | 1 |
| 3 | Silieiclastic sequence stratigraphy in well logs, cores, and outcrops: Con- cepts for high resolution correlation of time and facies显示文摘 | Van W J C Mitchum R M Campion K M et at | 1990 | AAPG Methods in Exploration1990，7，： | 1 |
| 4 | Silicielastic sequence stratigraphy in well logs, cores and outcrops : Concept for high-rsolution correlation of time and faeies显示文摘 | Van Wagone J C Mitchum R M Campion K M | 1990 | AAPG (Methods in Exploration Series)1990，7，： | 1 |
| 5 | Hip fractures in the elderly: A world-wide projection 显示文摘 | Cooper C Campion G Melton LJ | 1992 | Osteoporosis Int1992，2，6： | 1 |
| 6 | Siliciclastic sequence stratigraphy in well logs,cores and outcrops显示文摘 | Van Vagoner J C Mitchum R M Campion K M | 1990 | AAPG Methods in Exploration1990，7，1： | 1 |
| 7 | Hip fractures in the elderly:aworld wide projection显示文摘 | COOPER C CAMPION G MELTON L J | 1992 | Osteoporos Int1992，2，5： | 1 |
| 8 | Hip fractures in the elderly:a word wide projection显示文摘 | Campion G Melton LJ Ⅲ | 1992 | Osteporosis Int1992，2，： | 1 |
| 9 | Siliciclastic sequence stratigraphy in well logs,cores and out crops: Con cepts for high resolution correlation of time and facies显示文摘 | Van Wagoner J C Mitchum H M Campion K M | 1990 | AAPG Methods in Exploration Series1990，72，7： | 1 |
| 10 | On the role of charge-transfer resonances in the chemical mechanism of surface-enhanced Raman scattering 显示文摘 | Kambhampati P Child C M Campion A | 1996 | Journal of the Chemical Society-Faraday Transactions1996，92，23： | 1 |
| 11 | Silisiclastic sequence strtigraphy in well logs, Cores and outcrops: concepts for high-resolution correlation of time and facies显示文摘 | Van Wagoner J C Mitchum H M Campion K M | 1990 | AAPG Methods in Exploration Series1990，7，： | 1 |
| 12 | Relations between work group characteristics and effectiveness:implications for designing effective work groups显示文摘 | Campion M A Medsker G J & Higgs A C | 1993 | Personnel Psychology1993，46，4： | 1 |
| 13 | Siliciclastic sequence stratigraphy in welllogs , cores and outcrops 显示文摘 | Van Wagoner Mitchum J C Campion R M | 1990 | AAPG Methods in Exploration1990，，： | 1 |
| 14 | Hip fracturesin the elderly : a world-wide projection 显示文摘 | COOPER C CAMPION G MELTON L J 3RD | 1992 | Osteoporosisinternational : a journal established as result of cooperationbetween the European Foundation for Osteoporosis and theNational Osteoporosis Foundation of the USA1992，2，6： | 1 |
| 15 | Steel-concrete bond in lightweight fiber reinforced concrete under monotonic and cyclic actions 显示文摘 | Campione G Cucchiara C Mendola L L Papia M | 2005 | Engineering Structures2005，27，6： | 1 |
| 16 | Bone formation in a carbonate-substituted hydroxyapatite implant is inhibited by zoledronate:the importance of bioresorption to osteoconduction显示文摘 | Spence G Phillips S Campion C | | 0，，12： | 1 |
| 17 | Siliciclastic sequence stratigraphy in well log, cores, and outcrops : concepts for high-resolution correlation of time and facies 显示文摘 | Van Wagoner J C Mitchum R M Campion K M | 1990 | AAPG Methods in Exploration Series1990，7，： | 1 |
| 18 | Infammatory linear verrucous epidermal nevus and epilepsy: remission during valpmic acid therapy 显示文摘 | Diluvin L Campione E Mnrdenti C | 2013 | J Drugs Dermatol2013，12，1： | 1 |
| 19 | Hip fractures in the elderly:a world wide projection显示文摘 | Cooper C Campion G Melton LJ 3rd | 1992 | Osteoporos Int1992，，6： | 1 |
| 20 | Early-onset autosomal dominant Alzheimer disease:prevalence,genetic heterogeneity,and mutation spectrum显示文摘 | Campion D Dumanchin C Hannequin D | 1999 | Am J Hum Genet1999，65，3： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员