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| 1 | Involvement of sweet pepper Ca LOX2 in jasmonate-dependent induced defence against Western flower thrips显示文摘Insect herbivory can seriously hinder plant performance and reduce crop yield. Thrips are minute cell-content-feeding insects that are important vectors of viral plant pathogens, and are serious crop pests. We investigated the role of a sweet pepper (Capsicum annuum) lipoxygenase gene, CaLOX2, in the defense of pepper plants against Western flower thrips (Frankliniella occidentalis). This was done through a combination of in-silico, transcrip-tional, behavioral andchemicalanalyses.Our datashowthat CaLOX2 is involved in jasmonic acid (JA) biosynthesis and mediates plant resistance. Expression of the JA-related marker genes, CaLOX2 and CaPIN II, was induced by thrips feeding. Silencing of CaLOX2 in pepper plants through virus-induced gene silencing (VIGS) resulted in low levels of CaLOX2 transcripts, as well as significant reduction in the accumulation of JA, and its derivatives, upon thrips feeding compared to control plants. CaLOX2-silenced pepper plants exhibited enhanced susceptibility to thrips. This indicates that CaLOX2 mediates JA-dependent signaling, resulting in defenseagainstthrips. Furthermore,exogenous application of JA to pepper plants increased plant resistance to thrips, constrained thrips population development and made plants less attractive to thrips. Thus, a multidisciplinary approach shows that an intact lipoxygenase pathway mediates various components of sweet pepper defense against F. occidentalis. | Sandeep J Sarde Klaas Bouwmeester Jhon Venegas-Molina Anja David Wilhelm Boland Marcel Dicke | 2019 | Journal of Integrative Plant Biology2019,61,10: | 6 |
| 2 | Rhizobium Lipo-chitooligosaccharide Signaling Triggers Accumulation of Cytokinins in Medicago truncatula Roots显示文摘 | Arjan van Zeijl Rik H.M. Op den Camp Eva E. Deinum Tatsiana Charnikhova Henk Franssen Huub J.M. Op den Camp Harro Bouwmeester Wouter Kohlen Ton Bisseling Rene Geurts | 2015 | Molecular Plant2015,8,8: | 3 |
| 3 | SNARE-RNAi Results in Higher Terpene Emission from Ectopically Expressed Caryophyllene Synthase in Nicotiana benthamiana显示文摘植物生产众多的萜烯和许多努力被奉献了给萜烯 biosynthetic 基因的鉴定和描述。然而,很少萜烯怎么在房间以内并且从房间被搬运进 apoplast 被知道。为了在这调查泡熔化的一个通常认为的角色,处理,我们使用了 Agrobacterium 在 Nicotiana benthamiana 的调停 tumefaciens 的短暂 coexpression ? 有也的 MtVAMP721e-RNAi 构造(Vi ) caryophyllene synthase 或沉香醇 synthase 分别地。叶子的 Headspace 分析证明那 caryophyllene 或沉香醇排出物增加了关于五倍什么时候 N.? benthamiana VAMP72 功能被堵住。RNA 定序和 agroinfiltrated N. 的蛋白质 ubiquitination 分析 ? benthamiana 叶摘录建议增加的萜烯排出物可以被归因于 proteasome 基于三观察失灵:离开 DsRed 标记蛋白质的高水平, TPS+Vi (1 ) 出现了,(2 ) ubiquitinated 蛋白质的高水平,并且(3 ) 多重 proteasome 基因的协调导致的表示,大概由调停 proteasome 的反馈规定的缺乏引起了。然而, caryophyllene 或沉香醇没禁止 proteasome 相关的朊酶活动在在里面 ? vitro 试金。当结果不为在萜烯运输的泡熔化的一个角色是最后的时,他们确实显示出在泡熔化和某些萜烯的宫外的表示的抑制之间的一个强壮的相互作用。结果在新陈代谢的工程有潜在的应用。 | Hieng-Ming Ting Thierry L. Delatte Pim Kolkman Johana C. Misas-Villamil Renier A.L. van der Hoom Harro J. Bouwmeester Alexander R. van der Krol | 2015 | Molecular Plant2015,8,3: | 2 |
| 4 | Perichandral grafting for cartilage lesions of the knee显示文摘 | Homminga GN Bulstra SK Bouwmeester PS | 1990 | Jone Joint Surger1990,72,: | 1 |
| 5 | Experimental quantum teleportation显示文摘 | Bouwmeester D Pan Jian-Wei Mattle K | 1997 | Nature1997,390,: | 1 |
| 6 | Methods for estimation of the vacuum status in vacuumcircuit breakers 显示文摘 | Merck W F H Damstra G C Bouwmeester C E | 1999 | IEEE Transactions on Dielectrics and Electrical Insulation1999,6,4: | 1 |
| 7 | Amorpha-4,11- dienesvn- These: cloning and functional expression of a key enzyme in the biosynthetic pathway of the novel antimalaria drug artemisinin显示文摘 | Wallwaart T E Bouwmeester H J Hille J | 2001 | Planta2001,21,: | 1 |
| 8 | A ret respective analysis of two independent prospective cartilage repair studies:Autogenous perichondrial grafting versus subchondral drilling 10 years post surgery显示文摘 | Bouwmeester PS Kui JR Homminga GN | 2002 | J Ort hop Res2002,20,2: | 1 |
| 9 | Experimental Quantum Teleportation显示文摘 | Bouwmeester D Jian- Wei PAN etc | 1997 | Nature1997,,390: | 1 |
| 10 | Global approaches to protein-protein inte- ractions显示文摘 | Drewes G Bouwmeester T | 2003 | Current Opinion in Cell Biology2003,15,2: | 1 |
| 11 | Experimental Quantum Teleportation显示文摘 | BOUWMEESTER D PAN J W MATTLE K | 1997 | Nature1997,,390: | 1 |
| 12 | Effect of processing on the formation of biogenicamines andethyl carbamate in soybean tempe显示文摘 | Nout MJR Ruikes MMW Bouwmeester HM | 1993 | Journal of Food Safety1993,13,: | 1 |
| 13 | Simulation and assessment of wind array power variations based on simultaneous wind speed measurements显示文摘 | Schlueter R A Park G L Bouwmeester R | 1984 | IEEE Transactions on Power Apparatus and Systems1984,103,5: | 1 |
| 14 | Long term results of rib perichondriul grafts for repair of cartilage defects in the human knee显示文摘 | Bouwmeester SJ Beckers JM Kuijer R | 1997 | International Orthopaedics1997,21,5: | 1 |
| 15 | Experimental quantum teleportation 显示文摘 | BOUWMEESTER D PAN J W MATTLE K | 1997 | Nature1997,390,: | 1 |
| 16 | Experimental quantum teleportation显示文摘 | Bouwmeester D Pan J W | 1997 | Nature1997,390,: | 1 |
| 17 | Diagnostic Methods for Vacuum State Estimation显示文摘 | Damstra G C Merck W F H Bos P J Bouwmeester C E | 1998 | International Symposium on Discharges and Electrical Insulation in Vacuum ISDEIV1998,,2: | 1 |
| 18 | Phytophthora infestans isolates lacking class I ipiO va- riants are virulent on Rpi-blbl potato 显示文摘 | Champouret N Bouwmeester K Rietman H | 2009 | Molecular Plant-Microbe Interactions2009,22,: | 1 |
| 19 | Experimental quantum teleportation 显示文摘 | PAN MATTLE K | 1997 | Nature1997,390,: | 1 |
| 20 | Dense ceramic membranes for methane conversion显示文摘 | Bouwmeester H J M | 2003 | Catalysis Today2003,82,14: | 1 |