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| 1 | No evidence of HPV DNA in esophageal squamous cell carcinoma in a population of Southern Brazil显示文摘AIM:To investigate the association between human papillomavirus(HPV)and esophageal squamous cell carcinoma(ESCC)in southern Brazil.METHODS:We studied 189 esophageal samples from125 patients from three different groups:(1)102 biopsies from 51 patients with ESCC,with one sample from the tumor and another from normal esophageal mucosa distant from the tumor;(2)50 esophageal biopsies from 37 patients with a previous diagnosis of head and neck squamous cell carcinoma(HNSCC);and(3)37 biopsies from esophageal mucosa with normal appearance from 37 dyspeptic patients,not exposed to smoking or alcohol consumption.Nested-polymerase chain reaction(PCR)with the MY09/11 and GP5/6 L1primers was used to detect HPV L1 in samples fixed in formalin and stored in paraffin blocks.All PCR reactions were performed with a positive control(cervicovaginal samples),with a negative control(Human Genomic DNA)and with a blank reaction containing all reagents except DNA.We took extreme care to prevent DNA contamination in sample collection,processing,and testing.RESULTS:The histological biopsies confirmed the diagnosis of ESCC in 52 samples(51 from ESCC group and 1 from the HNSCC group)and classified as well differentiated(12/52,23.1%),moderately differentiated(27/52,51.9%)or poorly differentiated(7/52,13.5%).One hundred twenty-eight esophageal biopsies were considered normal(51 from the ESCC group,42 from the HNSCC group and 35 from dyspeptic patients).Nine had esophagitis(7 from the HNSCC and 2 from dyspeptic patients).Of a total of 189 samples,only 6 samples had insufficient material for PCR analysis:1 from mucosa distant from the tumor in a patient with ESCC,3from patients with HNSCC and 2 from patients without cancer.In 183 samples(96.8%)GAPDH,G3PDH and/orβ-globin were amplified,thus indicating the adequacy of the DNA in those samples.HPV DNA was negative in all the 183 samples tested:52 with ESCC,9 with esophagitis and 122 with normal esophageal mucosa.CONCLUSION:There was no evidence of HPV infection in different ESCC from southern Brazil. | Luís Carlos Moreira Antunes Joo Carlos Prolla Antonio de Barros Lopes Marta Pires da Rocha Renato Borges Fagundes | 2013 | World Journal of Gastroenterology2013，19，39： | 4 |
| 2 | Right ventricular function assessed by two-dimensional strain and tissue Doppler echocardiography in patients with pulmonary arterial hypertension and effect of vasodilator therapy显示文摘 | Borges AC Knebel F Eddicks S | 2006 | Am J Cardiol2006，98，4： | 1 |
| 3 | Correlation of VEGF production with IL - 1α and IL - 6 secretion by human pituitary adenoma cells 显示文摘 | BORG S A KERRY K E ROYDS J A | 2005 | European Journal of Endocrinology2005，152，： | 1 |
| 4 | Neurotrophins role in depression neurobiology:a review of basic and clinical ev-idence显示文摘 | Neto FL Borges G Torres-Sanchez S | 2011 | Curr Neuropharmacol2011，9，4： | 1 |
| 5 | Assistive technology in devel- oping countries: a review from the perspective of the Conven- tion on the Rights of Persons with Disabilities 显示文摘 | Borg J Lindstr6m A Larsson S | 2011 | Prosthet Or- thot Int2011，35，1： | 1 |
| 6 | Spatial and temporal variation of phytoplankton in two subtropical Brazilian reservoirs 显示文摘 | BORGES P A F TRAIN S RODRIGUES L C | 2008 | Hydrobiologia2008，607，： | 1 |
| 7 | ERBIN:A basolateral PDZ protein that interacts with the mammalian ERBB2/HER2 receptor显示文摘 | Borg JP Marchetto S Le Bivic A | 2000 | Nature Cell Biol2000，2，： | 1 |
| 8 | Teacher cognition in language teaching: A review of research on what language teachers think, know, believe and do 显示文摘 | Borg S | 2003 | Language Teaching2003，36，： | 1 |
| 9 | Biochemical and genetic characterization of the pathways for trehalose metabolism in Propionibacterium freudenreichii, and their role in stress response显示文摘 | Cardoso F S Castro R Borges N | 2007 | Microbiology2007，153，1： | 1 |
| 10 | Glycoprotein glycosyltransferaseactivities in serum in alcohol-abusing patients andhealthy controls显示文摘 | Stibler H Borg S | 1991 | Scand J Clin Lab Invest1991，51，1： | 1 |
| 11 | IL - 6 is associated with pituitary tumour invasiveness 显示文摘 | SULIMAN M BORG S KERRY K | 2001 | Endocrine Pathology2001，12，： | 1 |
| 12 | Identifying improvements in water management of bus-washing stations in Brazil显示文摘 | Almeida C M V B Borges J D Bonilla S H | 2010 | Resources Conservation and Recycling2010，54，11： | 1 |
| 13 | Percutaneous plating of distal tibia1 fractures preliminary results in 21 patients 显示文摘 | Borg T Larson S Lindsjo U | 2004 | Injury2004，35，： | 1 |
| 14 | Quantitative effect of CYP2D6 genotype and inhibitors on tamoxifen metabolism:implication for optimization of breast cancer treatment显示文摘 | Borges S Desta Z Li L | 2006 | Clin Pharmacol Ther2006，80，1： | 1 |
| 15 | Quantitative effect of CYP2D6 genotype and inhibitors on tamoxifen metabolism : implication for optimization of breast cancer treatment显示文摘 | Borges S Desta Z Li L | 2006 | Clin Pharmacol Ther2006，80，： | 1 |
| 16 | Cavitation of pulmonary metastases as aresponse to chemotherapy 显示文摘 | Thalinger A R Rosenthal S N Borg S | 1980 | Cancer1980，46，6： | 1 |
| 17 | ERBIN: a basolateral PDZ protein that interacts with the mamma- lian ERBB2/HER2 receptor显示文摘 | Borg JP Marchetto S Le Bivic A | 2000 | Nat Cell Biol2000，2，7： | 1 |
| 18 | Identification of new protein species among 33 different small GTP-binding proteins encoded by cDNAs from Lotus japonicus and expression of corresponding mRNAs in developing root nodules显示文摘 | Borg S Brandstrup B Jensen TJ | 1997 | Plant J1997，11，： | 1 |
| 19 | Analysis of long- range transport influences on urban PM10 using two-stage atmospheric trajectory clusters显示文摘 | Borge R Lumbreras J Vardoulakis S | 2007 | Atmospheric Environment2007，41，21： | 1 |
| 20 | Teacher cognition in language teaching: A review of research on what language teachers think, know, believe, and do 显示文摘 | Borg S | 2003 | Language Teaching2003，36，2： | 1 |
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