|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Analysis of hepatitis B virus preS1 variability and prevalence of the rs2296651 polymorphism in a Spanish population显示文摘AIM To determine the variability/conservation of the domain of hepatitis B virus(HBV) pre S1 region that interacts with sodium-taurocholate cotransporting polypeptide(hereafter, NTCP-interacting domain) and the prevalence of the rs2296651 polymorphism(S267 F, NTCP variant) in a Spanish population. METHODS Serum samples from 246 individuals were included and divided into 3 groups: patients with chronic HBV infection(CHB)(n = 41, 73% Caucasians), patients with resolved HBV infection(n = 100, 100% Caucasians) and an HBV-uninfected control group(n = 105, 100% Caucasians). Variability/conservation of the amino acid(aa) sequences of the NTCPinteracting domain,(aa 2-48 in viral genotype D) and a highly conserved pre S1 domain associated with virion morphogenesis(aa 92-103 in viral genotype D) were analyzed by next-generation sequencing and compared in 18 CHB patients with viremia > 4 log IU/mL. The rs2296651 polymorphism was determined in all individuals in all 3 groups using an in-house real-time PCR melting curve analysis.RESULTS The HBV pre S1 NTCP-interacting domain showed a high degree of conservation among the examined viral genomes especially between aa 9 and 21(in the genotype D consensus sequence). As compared with the virion morphogenesis domain, the NTCPinteracting domain had a smaller proportion of HBV genotype-unrelated changes comprising > 1% of the quasispecies(25.5% vs 31.8%), but a larger proportion of genotype-associated viral polymorphisms(34% vs 27.3%), according to consensus sequences from Gen Bank patterns of HBV genotypes A to H. Variation/conservation in both domains depended on viral genotype, with genotype C being the most highly conserved and genotype E the most variable(limited finding, only 2 genotype E included). Of note, proline residues were highly conserved in both domains, and serine residues showed changes only to threonine or tyrosine in the virion morphogenesis domain. The rs2296651 polymorphism was not detected in any participant.CONCLUSION In our CHB population, the NTCP-interacting domain was highly conserved, particularly the proline residues and essential amino acids related with the NTCP interaction, and the prevalence of rs2296651 was low/null. | Rosario Casillas David Tabernero Josep Gregori Irene Belmonte Maria Francesca Cortese Carolina González Mar Riveiro-Barciela Rosa Maria López Josep Quer Rafael Esteban Maria Buti Francisco Rodríguez-Frías | 2018 | World Journal of Gastroenterology2018，24，6： | 5 |
| 2 | Expression of connexins 36,43, and 45 during postnatal development of the mouse retina显示文摘 | Kiahara AH Mantovani de Castro L Belmonte MA Yan CY Moriscot AS Hamassald DE | 2006 | J Neurobiol2006，66，13： | 1 |
| 3 | Monitoring of pesticides in agricultural water and soil samples from Andalusia by liquid chromatography coupled to mass spectrometry 显示文摘 | Belmonte Vega A Garrido Frenich A Martfnnez Vidal J L | 2005 | Analytical Chimica Acta2005，538，： | 1 |
| 4 | Determina- tion of five antifouling agents in water by gas chroma- tography with positive/negative chemical ionization and tandem mass spectrometric detection 显示文摘 | Hemando M D Piedra L Belmonte A | 2001 | Journal of Chromatography A2001，938，： | 1 |
| 5 | Expression of connexins 36, 43, and 45 during postnatal development of the mouse retina显示文摘 | Kihara AH Mantovani de Castro L Belmonte MA | 2006 | J Neurobiol2006，66，13： | 1 |
| 6 | Behaviour of molecular weight distribution for the liquid fraction of pig slurry treated by anaerobic digestion 显示文摘 | Rodirguez D C Belmonte M Penuela G Campos J L Vidal G | 2011 | Environmental Technology2011，33，4： | 1 |
| 7 | Echocardiographic Evaluation of Asymptomatic Parental and Sibling Cardiovascular Anomalies Associaed With Congenital Left Ventricular Outflow Tract Lesions显示文摘 | Lewin B Mcbride L Belmont W | 2004 | Pediatrics2004，114，3： | 1 |
| 8 | Determination of traces of five antifouling agents in water by gas chromatography with positive/negative chemical ionisation and tandem mass spectrometric detection显示文摘 | Hernando M D Piedra L Belmonte A Aguera A Fernandez-Alba A R | | 0，，： | 1 |
| 9 | Application of internal quality control to the analysis of quaternary ammonium compounds in surface and groundwater from Andalusia (Spain) by liquid chromatography with mass spectrometry显示文摘 | J L Mart'lnez Vidal A Belmonte Vega F J S anchez L opez | 2004 | Journal of Chromatography A2004，1050，： | 1 |
| 10 | Effects of seeding and amounts of Y2O3 : Al2O3 additives on grain growth in Si3N4 ceramics 显示文摘 | Belmonte M Pablos D L Osendi M I | 2008 | Materials Science and Engineering A2008，475，12： | 1 |
| 11 | The effect of reduced glutathione on morphology and gene expression of white spruce (Picea glauca) somatic embryos显示文摘 | Stasolla C Belmonte MF Zyl L | 2004 | Journal of Experimental Botany2004，55，397： | 1 |
| 12 | The extracellular signal-regulated kinase isoform ERK1 is specifically required for in vitro and in vivo adipogenesis显示文摘 | Bost F Aouadi M Caron L Even P Belmonte N Prot M Dani C Hofman P Pagès G P | | 0，，02： | 1 |
| 13 | HIV-1 infection and chemokine receptor modulation 显示文摘 | RUIBAL-ARES BH BELMONTE L BARE PC | 2004 | Curr HIV Res2004，2，1： | 1 |
| 14 | Survival,mortality and causes of death in inflammatory myopathies显示文摘 | Torres C Belmonte R Carmona L | | 0，，03： | 1 |
| 15 | Survival, mortality and causes of death in in- flammatory myopathies显示文摘 | Torres C Belmonte R Carmona L | 2006 | Autoimmunity2006，39，： | 1 |
| 16 | The intestinal mucosa as a reservoir of HIV-1 infection after successful HAART 显示文摘 | Belmonte L Olmos M Fanin A | 2007 | AIDS2007，21，： | 1 |
| 17 | Mycobacterium boris BCG Toll-like receptors 2 and 4 cooperation increases the innate epithelial immune response显示文摘 | Me ndez-Samperio P Belmont L Miranda E | 2008 | Arch Med Res2008，39，1： | 1 |
| 18 | Pulmonary epi -thelioid hemangioendothelioma and bevacizumab 显示文摘 | Belmont L Zemoura L Couderc L J | 2008 | J ThoracOncol2008，3，5： | 1 |
| 19 | The intestinal mucosa as a reservoir of HIV-1 infection after successful HAART显示文摘 | Belmonte L Olmos M Fanin A | 2007 | AIDS2007，21，15： | 1 |
| 20 | Autism as a dis- order of neural information processing: directions for re- search and targets for therapy显示文摘 | Belmonte MK Cook EH Jr Anderson et a l | 2004 | Molecular Psychiatry2004，9，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员