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| 1 | 黑曲霉葡萄糖氧化酶基因的克隆及其在酵母中的高效表达显示文摘将黑曲霉葡萄糖氧化酶 (GOD)基因重组进大肠杆菌 酵母穿梭质粒pPIC9,转化甲基营养酵母Pichiapasto risGS115 ,构建出GOD的高产酵母工程菌株。在酵母α Factor及AOX1基因启动子和终止信号的调控下 ,黑曲霉GOD在甲基酵母中大量表达并分泌至胞外 ,经甲醇诱导 3~ 4d ,发酵液中的GOD活力可达 30~ 40u mL。SDS PAGE证实GOD在培养物上清中的含量显著高于其它杂蛋白 ,约占胞外蛋白总量的 6 0 %~ 70 % ,经QSepharoseTM FastFlow离子交换柱一步纯化即达电泳纯。重组酵母GOD比活达 42 6 6 3u mg蛋白 ,是商品黑曲霉GOD的 1 6倍。动力学性质分析表明 ,重组酵母GOD的Km 和kcat分别为 38 2 5mmol L和 34 92 6 6s- 1 ,与商品黑曲霉GOD相比 ,具有更高的催化效率。重组酵母GOD的高活力特性可有效提高葡萄糖传感器的线性检测范围。 | 周亚凤 张先恩 刘虹 张成刚 Anthony E G Cass | 2001 | 生物工程学报2001，17，4： | 25 |
| 2 | Spectrum of anemia associated with chronic liver disease显示文摘Anemia of diverse etiology is a common complication of chronic liver diseases. The causes of anemia include acute or chronic gastrointestinal hemorrhage, and hypersplenism secondary to portal hypertension. Severe hepatocellular disease predisposes to hemorrhage because of impaired blood coagulation caused by deficiency of blood coagulation factors synthesized by hepatocytes, and/or thrombocytopenia. Aplastic anemia, which is characterized by pancytopenia and hypocellular bone marrow, may follow the development of hepatitis. Its presentation includes progressive anemia and hemorrhagic manifestations. Hematological complications of combination therapy for chronic viral hepatitis include clinically signif icant anemia, secondary to treatment with ribavirin and/or interferon. Ribavirininduced hemolysis can be reversed by reducing the dose of the drug or discontinuing it altogether. Interferons may contribute to anemia by inducing bone marrow suppression. Alcohol ingestion is implicated in the pathogenesis of chronic liver disease and may contribute to associated anemia. In patients with chronic liver disease, anemia may be exacerbated by defi ciency of folic acid and/or vitamin B12 that can occur secondary to inadequate dietary intake or malabsorption. | Rosario Gonzalez-Casas E Anthony Jones Ricardo Moreno-Otero | 2009 | World Journal of Gastroenterology2009，15，37： | 13 |
| 3 | Liver disease and erythropoietic protoporphyria:A concise review显示文摘The porphyries are a group of metabolic disorders characterized by deficiencies in the activity of enzymes involved in the biosynthesis of heme.In erythropoietic protoporphyria(EPP),in the majority of cases an autosomal dominant disease,there is a mutation of the gene that encodes ferrochelatase(FECH).FECH deficiency is associated with increased concentrations of protoporphyrin in erythrocytes,plasma,skin and liver.The prevalence of this inherited disorder oscillates between 1:75 000 and 1:200 000.Clinical manifestations of EPP appear in early infancy upon first exposure to the sun.Nevertheless,approximately 5%-20% of patients with EPP develop liver manifestations.Retention of protoporphyrin in the liver is associated with cholestatic phenomena and oxidative stress that predisposes to hepatobiliary disease of varying degrees of severity,such as cholelithiasis,mild parenchymal liver disease,progressive hepatocellular disease with end-stage liver disease and acute liver failure.Liver damage is the major risk in EPP patients,so surveillance and frequent clinical and biochemical liver follow-up is mandatory.The diagnostic approach consists in detecting increased levels of protoporphyrin,decreased activity of FECH and genetic analysis of the FECH gene.A variety of nonsurgical therapeutic approaches have been adopted for the management of EPP associated with liver disease,but none of these has been shown to be unequivocally efficacious.Nevertheless,some may have a place in preparing patients for liver transplantation.Liver transplantation does not correct the constitutional deficiency of FECH.Consequently,there is a risk of recurrence of liver disease after liver transplantation as a result of continuing overproduction of protoporphyrin.Some authors recommend that bone marrow transplantation should be considered in liver allograft recipients to prevent recurrence of hepatic disease. | María José Casanova-González María Trapero-Marugán E Anthony Jones Ricardo Moreno-Otero | 2010 | World Journal of Gastroenterology2010，16，36： | 7 |
| 4 | Current status of imaging in nonalcoholic fatty liver disease显示文摘Non-alcoholic fatty liver disease(NAFLD) is the most common diffuse liver disease, with a worldwide prevalence of 20% to 46%. NAFLD can be subdivided into simple steatosis and nonalcoholic steatohepatitis. Most cases of simple steatosis are non-progressive, whereas nonalcoholic steatohepatitis may result in chronic liver injury and progressive fibrosis in a significant minority. Effective risk stratification and management of NAFLD requires evaluation of hepatic parenchymal fat, fibrosis, and inflammation. Liver biopsy remains the current gold standard; however, non-invasive imaging methods are rapidly evolving and may replace biopsy in some circumstances. These methods include well-established techniques, such as conventional ultrasonography, computed tomography, and magnetic resonance imaging and newer imaging technologies, such as ultrasound elastography, quantitative ultrasound techniques, magnetic resonance elastography, and magnetic resonancebased fat quantitation techniques. The aim of this article is to review the current status of imaging methods for NAFLD risk stratification and management, including their diagnostic accuracy, limitations, and practical applicability. | Qian Li Manish Dhyani Joseph R Grajo Claude Sirlin Anthony E Samir | 2018 | World Journal of Hepatology2018，10，8： | 6 |
| 5 | Characterisation and seasonal variations of particles in the atmosphere of rural, urban and industrial areas:Organic compounds显示文摘Atmospheric aerosol samples(PM_(2.5–0.3), i.e., atmospheric particles ranging from 0.3 to2.5 μm) were collected during two periods: spring–summer 2008 and autumn–winter 2008–2009, using high volume samplers equipped with cascade impactors. Two sites located in the Northern France were compared in this study: a highly industrialised city(Dunkirk) and a rural site(Rubrouck). Physicochemical analysis of particulate matter(PM) was undertaken to propose parameters that could be used to distinguish the various sources and to exhibit seasonal variations but also to provide knowledge of chemical element composition for the interpretation of future toxicological studies. The study showed that PM2.5–0.3concentration in the atmosphere of the rural area remains stable along the year and was significantly lower than in the urban or industrial ones, for which concentrations increase during winter.High concentrations of polycyclic aromatic hydrocarbons(PAHs), dioxins, furans and dioxin like polychlorinated biphenyls(DL-PCBs), generated by industrial activities, traffic and municipal wastes incineration were detected in the samples. Specific criteria like Carbon Preference Index(CPI) and Combustion PAHs/Total PAHs ratio(CPAHs/TPAHs) were used to identify the possible sources of atmospheric pollution. They revealed that paraffins are mainly emitted by biogenic sources in spring–summer whereas as in the case of PAHs, they have numerous anthropogenic emission sources in autumn-winter(mainly from traffic and domestic heating). | Fabrice Cazier Paul Genevray Dorothée Dewaele Habiba Nouali Anthony Verdin Frédéric Ledoux Adam Hachimi Lucie Courcot Sylvain Billet Saad Bouhsina Pirouz Shirali Guillaume Garcon Dominique Courcot | 2016 | Journal of Environmental Sciences2016，28，6： | 4 |
| 6 | Twenty-five years of research on the effects of exercise training in breast cancer survivors:A systematic review of the literature显示文摘AIM:To investigate the role of exercise training the past 25 years on major physiological-psychological outcomes studied thus far in this patient population.METHODS:Pub Med, Medline Plus, the Cochrane Library, Web of Science, SportD iscus, Embase, Scorpus, and Google Scholar were searched from September to November 2013 to identify exercise training studies that used objective measurements of fitness and/or patient reported outcomes assessed pre and post-exercise training with statistical analyses performed in at least one of the following outcome measurements:Cardiorespiratory function, body composition, muscular strength, fatigue, depression, and overall quality of life. Five reviewers independently identified the studies that met the criteria for the review and discrepancies were resolved by consensus among all authors.RESULTS:Fifty-one studies were included in this review with 5 from the period between 1989-1999, 11 from 2000-2006, and 35 from 2007-2013. The evolution of study designs changed from aerobic only exercise training interventions(1989-1999), to a combination of aerobic and resistance training(2000-2006), to studies including an arm of resistance training or examining the effects of resistance training as the main mode of exercise(2007-2013). Overall, the benefits of exercise showed improvements in cardiorespiratory function, body composition, strength, and patient reported outcomes including fatigue, depression, and quality of life.CONCLUSION:Exercise training appears to be safe for most breast cancer patients and improvements in physiological, psychological, and functional parameters can be attained with regular participation in moderate intensity exercise. | Claudio L Battaglini Robert C Mills Brett L Phillips Jordan T Lee Christina E Story Marcelo GB Nascimento Anthony Carl Hackney | 2014 | World Journal of Clinical Oncology2014，5，2： | 3 |
| 7 | Prospective and long-term evaluation of erect penile length obtained with inflatable penile prosthesis to that induced by intracavernosal injection显示文摘在有那的可膨胀的阴茎修复术(IPP ) 培植在外科前由 in-tracavernosal 注射(帝国化学工业公司) 导致了以后,我们比较了直立的阴茎长度。有充分勃起的 11 个病人的一个总数在阴茎颜色的时候由 Trimix (papaverine, phentolamine 和前列腺素 E1 ) 的 0.25 mL 的帝国化学工业公司导致了双 Doppler ultrasonography 在这研究被注册。病人随后为他们的可勃起的机能障碍(编辑) 作为最终的治疗经历了 IPP 培植。对 IPP 培植作为结果的直立的阴茎长度在 6 个星期被测量, 6 个月并且在在外科以后的 1 年。为男人(填隙用木片) 的性健康库存在 IPP 培植以后被管理在前,并且在 6 个月和 1 年。是导致了由树立阴茎长度(吝啬的 s.e ) 而与 IPP 达到的长度是 12.4,帝国化学工业公司是 13.2 0.4 厘米 0.3,在第六个星期,第六个月和 1 年的后续的 12.5 0.3 和 12.5 0.4 厘米分别地。有 0.83 0.25,分别地, 0.75 0.20 和 0.74 0.15 厘米在 IPP 培植以后在 6 个星期, 6 个月和 1 年在直立的阴茎长度减少什么时候与那相比在帝国化学工业公司以后(P < 0.05 ) 。没有抱怨,为报导更短的阴茎的病人的填隙用木片分数与为病人的那些一样(P > 0.05 ) 。到我们的知识,这是第一研究客观地在 IPP 培植以后在直立的阴茎长度看重要减少是否与那相比在帝国化学工业公司以后。然而,这减少没在对待编辑影响 IPP 的有效性。 | Run Wang Galen E Howard Anthony Hoang Jiu-Hong Yuan Hao-Cheng Lin Yu-Tian Dai | 2009 | Asian Journal of Andrology2009，11，4： | 3 |
| 8 | Non-alcoholic fatty liver disease in the Philippines:Comparable with other nations?显示文摘AIM: To evaluate the prevalence and determined the common characteristics of patients diagnosed with non- alcoholic fatty liver disease (NAFLD) at the Philippine General Hospital, Manila, from January 1999 to December 2004. METHODS: NAFLD was diagnosed in 134 from a total of 1102 patients, based on clinical, ultrasonographic and/or histopathological findings. Patients with conditions associated with secondary NAFLD were excluded. Chart review was done to note demographics, co- morbid illnesses, physical characteristics, hepatomegaly, aspartate/alanine aminotransferase (AST/ALT) levels, albumin, lipid levels, alkaline phosphatase, prothrombin time, and partial thromboplastin time. Data obtained were analyzed using the statistical program SPSS version 10. RESULTS: Of the 134 patients included, 71% were female and 29% male. Mean patient age was 42.2 years. Sixty percent of patients were obese, 56% had hepatomegaly, and 69% had diabetes. AST levels were elevated in 45% of subjects and ALT levels in 64%. CONCLUSION: The prevalence of NAFLD at our institution was 12.2%. Patients diagnosed appear to be younger in age in contrast to previous studies. Female sex, obesity, elevated liver enzymes, and diabetes were characteristic features of our NAFLD patients, which is comparable to previous studies from other countries. | Mark Anthony A De Lusong E Labio L Daez V Gloria | 2008 | World Journal of Gastroenterology2008，14，6： | 3 |
| 9 | Acquired mutation of the tyrosine kinase JAK2 in human myeloproliferative disorders显示文摘 | E Joanna Baxter Linda M Scott Peter J Campbell Clare East Nasios Fourouclas Soheila Swanton George S Vassiliou Anthony J Bench Elaine M Boyd Natasha Curtin Mike A Scott Wendy N Erber Anthony R Green | 2005 | The Lancet . 2005 (9464)2005，，： | 2 |
| 10 | 2008 estimate of worldwide rotavirus-associated mortality in children younger than 5 years before the introduction of universal rotavirus vaccination programmes: a systematic review and meta-analysis显示文摘 | Jacqueline E Tate Anthony H Burton Cynthia Boschi-Pinto A Duncan Steele Jazmin Duque Umesh D Parashar | 2012 | The Lancet Infectious Diseases2012，，2： | 2 |
| 11 | 2007 Guidelines for the Management of Arterial Hypertension: The Task Force for the Management of Arterial Hypertension of the European Society of Hypertension (ESH) and of the European Society of Cardiology (ESC)显示文摘 | Giuseppe Mancia Guy De Backer Anna Dominiczak Renata Cifkova Robert Fagard Giuseppe Germano Guido Grassi Anthony M Heagerty Sverre E Kjeldsen Stephane Laurent Krzysztof Narkiewicz Luis Ruilope Andrzej Rynkiewicz Roland E Schmieder Harry AJ Struijker Boudi | 2007 | Journal of Hypertension2007，，6： | 2 |
| 12 | 2007 Guidelines for the Management of Arterial Hypertension: The Task Force for the Management of Arterial Hypertension of the European Society of Hypertension (ESH) and of the European Society of Cardiology (ESC)显示文摘 | Giuseppe Mancia Guy De Backer Anna Dominiczak Renata Cifkova Robert Fagard Giuseppe Germano Guido Grassi Anthony M Heagerty Sverre E Kjeldsen Stephane Laurent Krzysztof Narkiewicz Luis Ruilope Andrzej Rynkiewicz Roland E Schmieder Harry AJ Struijker Boudi | 2007 | Journal of Hypertension2007，，6： | 2 |
| 13 | Solubilities and thermodynamic properties of gases in the ionic liquid 1-n-butyl-3-methylimidazolium hexafluorophosphate显示文摘 | Anthony J L Maginn E J Brennecke J F | 2002 | J Phys Chem B2002，106，29： | 1 |
| 14 | Storm-generated morphological change and longshore sand transport in the intertidal zone of multi-barred macrotidal beach显示文摘 | Sedrati M Anthony E J | 2007 | Marine Geology2007，244，： | 1 |
| 15 | Clean combustion of solid fuels显示文摘 | Wang J S Anthony E J | 2008 | Applied Energy2008，85，： | 1 |
| 16 | Promoting and Countering Con- sumer Misconceptions of Random Events: The Case of Perceived Control and State-Sponsored Lotteries显示文摘 | Anthony M Anne M David E | 2001 | Journal of Public Policy Marketing2001，20，2： | 1 |
| 17 | Sorbent cost and performance in CO2 capture systems显示文摘 | Abanades J C Rubin E S Anthony E J | 2004 | Industrial & Engineering ChemistryResearch2004，43，13： | 1 |
| 18 | the cytokine and chemokine expression profile of nucleus pul- posus cells:implications for degeneration and regeneration of the intervertebral disc显示文摘 | Kate L E Phillips Nell Chiverton Anthony LR Michael | 2013 | Arthritis Res Ther2013，15，6： | 1 |
| 19 | Prevention and Control of Influenza with Vaccines Recommendations of the Advisory Committee on Immunization Practices (ACIP), 2010显示文摘 | Fiore Anthony E Uyeki Timothy M Broder Karen Finelli Lyn Euler Gary L Singleton James A Iskander John K Wortley Pascale M Shay David K Bresee Joseph S Cox Nancy J | 2010 | MMWR. Morbidity and Mortality Weekly Report2010，，： | 1 |
| 20 | Sources of sedimentary lipids deduced from stable carbon isotope analyses of individual compounds 显示文摘 | Gareth Rieley Robert J Collier David M Jones Geoffrey Eglinton Paul A Eakin Anthony E Fallick | 1991 | Nature1991，352，： | 1 |
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