|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Chinese Economic Structure and SDA Model | CHEN Xi\|kang 1,\ GUO Ju\|e 2 1. Academy of Machemation and Systems Science, Chinese Academy of Science Beijing 100080, China 2. Shaanxi Institute of Finance and Economics, Xi′an 710061, China | 2000 | Systems Science and Systems Engineering2000，10，2： | 40 |
| 3 | OPTICAL PROPERTIES OF GeO_2 AND Ge NANOCRYSTALS显示文摘he co m pound m aterial of n m size particles Ge O2 Si O2 w as synthesied through hydrolysis of Si( O C2 H4) and Ge Cl4 . A heat treatm ent w as carried out for the sa m ples at 100 ~1200 ℃in air . Its optical property w as deter mined by U V Vis spectur m . We have found that theabsorption edge of spectru m shifted progressively to longer w avelengths . The quantu m size ef fect of nanocrystals appears because crystals gro w and energy of optical band gap reduces d ueto the influence of te m perature . By the analysis of X ray diffraction w e have observed theprocess in w hich the structure of particles changed fro m disorder into order . | Y.H.Zhou 1) ,Y.Y.Feng 2) and H.Y.Lu 2) 1)Department of Physics Nanjing Normal University, Nanjing 210097,China 2)Physics and Ch寁O P T I C A L P R O P E R T I E S O F Ge O2 A N D Ge N A N O C R Y S T A L S Y. H. Zhou1) , Y. Y. Feng2) | 1999 | Acta Metallurgica Sinica(English Letters)1999，12，5： | 22 |
| 4 | Study of heteroserum-induced rat liver fibrosis model and its mechanism显示文摘StudyofheteroseruminducedratliverfibrosismodelanditsmechanismHUANGZhiGang,ZHAIWeiRong,ZHANGYueEandZHANGXiuRongSubjecthea... | HUANG Zhi Gang, ZHAI Wei Rong, ZHANG Yue E and ZHANG Xiu Rong | 1998 | World Journal of Gastroenterology1998，4，3： | 22 |
| 5 | Comparison of Transformation Efficiency of piggyBac Transposon among Three Different Silkworm Bombyx mori Strains显示文摘在由编码 transposase 定序的小猪用绳子拖的平底渡船向量和助手 plasmidcontaining 的混合物的微注射编码基因进蚕鸡蛋的提高的绿荧光蛋白质(EGFP ) 的介绍以后比较的蚕 Bombyx moriwas 的三不同紧张的转变率。尽管在在在微注射鸡蛋(P=0.1258 ) 的肥沃的蛾的百分比的三紧张之中没有重要差别, G0 的百分比转变了在肥沃的蛾和注射鸡蛋的蛾两个都是显著地不同的(P=0.01368 和 P=0.02398,分别地) 。Nistari 紧张(印度紧张) 的转变率比另外的二紧张, Golden-yellow-cocoon (Vietnamesestrain ) 和 Jiaqiu (中国紧张) 的显著地高,它有类似的率。这些结果显示基于 piggyBac 的系统的转变效率可能与不同基因背景与蚕紧张变化。内长的象 piggyBac 一样元素的存在可能是影响介绍 piggyBac-derivedvectors,和多样的数量的转变效率的一个重要因素,在不同的蚕紧张的激活可能说明重要差别。 | Boxiong ZHONG~1 Jianying LI~1 Jin’e CHEN~(1,2) Jian YE~1 and Songdong YU~1 ~1College of Animal Sciences,Zhejiang University,Hangzhou 310029,China ~2Institute of Sericultural Research,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China | 2007 | Acta Biochimica et Biophysica Sinica2007，39，2： | 17 |
| 6 | Expression of calreticulin is associated with infiltration of T-cells in stage ⅢB colon cancer显示文摘AIM: To investigate the correlation between expression of calreticulin and infiltration of lymphocytes in stage ⅢB colon cancer. METHODS: Sixty-eight pathologically-confirmed speci-mens were obtained from stage ⅢB (T3N1M0) colon cancer patients who underwent radical resection between January 1999 and May 2002 at the Cancer Center of Sun Yat-Sen University, Guangzhou, China. Immuno-histochemical analysis was performed to show infiltration of lymphocytes and expression of calreticulin in colon cancer. Association between calreticulin expression, infiltration of lymphocytes, and 5-year survival rate of patients was assessed. RESULTS: The expression level of calreticulin was lower in cancer nest than in its adjacent normal epithelium since 61.8% (42/68) of the samples were stained with calreticulin in colon cancer. The expression of calreticulin in colon cancer was associated with the infi ltration of CD45RO+ cells rather than with that of CD3+ cells. In addition, the stronger expression of calreticulin and the higher infiltration of CD3+ and CD45RO+ cells in colon cancer were associated with the higher 5-year survival rate of patients. CONCLUSION: Expression of calreticulin is associated with infiltration of T-cells, which implies that a low expression level of molecular marker may represent a new mechanism underlying immune escape in colon cancer. | Rui-Qing Peng, Ya Ding, Xing Zhang, Yi-Xin Zeng, Xiao- Shi Zhang, State Key Laboratory of Oncology in South China, Biotherapy Center, Cancer Center, Sun Yat-Sen University, 651 Dongfeng R E, Guangzhou 510060, Guangdong Province, China Ying-Bo Chen, Zhi-Wei Zhou, State Key Laboratory of Oncology in South China, Department of Gastric and Pancreatic Oncology, Cancer Center, Sun Yat-Sen University, 651 Dongfeng R E, Guangzhou 510060, Guangdong Province, China Rong Zhang, State Key Laboratory of Oncology in South China, Department of Endoscopy, Cancer Center, Sun Yat-Sen University, 651 Dongfeng R E, Guangzhou 510060, Guangdong Province, China Xing-Juan Yu, Yi-Xin Zeng, State Key Laboratory of Oncology in South China, Department of Experimental Research, Cancer Center, Sun Yat-Sen University, 651 Dongfeng R E, Guangzhou 510060, Guangdong Province, China | 2010 | World Journal of Gastroenterology2010，16，19： | 16 |
| 7 | Down-Regulation of CD44 Contributes to the Differentiation of HL-60 Cells Induced by ATRA or HMBA显示文摘CD44 is highly expressed in human acute myeloid leukemia (AML) cells. Some experiments had shown that it was possible to reverse differentiation blockage in AML cells by CD44 ligation with specific antibodies, indicating that CD44 was closely related to the differentiation of leukemia cells. The differentiation of acute promyelocytic leukemia cell line HL-60 cells could be induced by all trans-retinoic acid (ATRA) and hexamethylene bisacetamide (HMBA), but so far the mechanism was not demonstrated clearly. In the present study, we investigated whether ATRA or HMBA induced the growth arrest of HL-60 cells by down-regulating the expression of CD44. The results indicated that the proliferation of HL-60 cells was obviously inhibited and the differentiation was induced by both ATRA and HMBA. The decreased expression of CD44 and cyclin E mRNA, and the increased expression of p27 and p21 at mRNA levels were observed. Furthermore, there was a negative correlation between the expression of CD44 and p27. It was concluded that ATRA and HMBA played a role in the differentiation induction of HL-60 cells, which was mediated by the down-regulation of CD44, accompanied by down-regulation of cyclin E, and up-regulation of p27 and p21 mRNA. | Jianing Liu1, Gaofeng Bi1, Pei’e Wen1, Weihua Yang1, Xia Ren1, Tianhua Tang1, Chao Xie1, Wei Dong1 and Guosheng Jiang1, 21Department of Hemato-oncology, Institute of Basic Medicine, Shandong Academy of Medical Sciences Key Laboratory for Modern Medicine and Technology of Shandong Province Key Medical Laboratory for Tumor Immunology & Traditional Chinese Medicine Immunology of Shandong Province, Jinan, Shandong 250062, China | 2007 | Cellular & Molecular Immunology2007，4，1： | 12 |
| 8 | Boundary Layer Theory and the Zero-Viscosity Limit of the Navier-Stokes Equation | Weinan E Department of Mathematics and Program in Applied and Computational Mathematics,Princeton University,USA | 2000 | Acta Mathematica Sinica,English Series2000，16，2： | 10 |
| 9 | High-accuracy topographical information extraction based on fusion of ASTER stereo-data and ICESat/GLAS data in Antarctica显示文摘In order to better support Antarctic inland ice sheet expedition from Zhongshan Station to Dome A,the topographic data are necessary.At present,although the entire Antarctic DEM provided by RAMP(Ra-darsat Antarctic Mapping Project) was estimated at the highest horizontal(spatial) resolution of about 200 m,the real horizontal resolution of the DEM varies from place to place depending on the density and scale of the original source data.For ice shelves and the inland ice sheet,the horizontal resolution is about 5 km;the vertical accuracy is estimated to be ±50 m in interior East Antarctic ice sheet and away from the mountain ranges.Therefore,more accurate topographic data are unavailable in Antarc-tica.In order to meet the requirements of high-accuracy topographic information for further researches,this paper mainly addresses a fusion study of ASTER stereo pairs and ICESat/GLAS altimetry data for extraction of high-accuracy DEM in East Antarctica,based on the high horizontal resolution(15 m) of ASTER and vertical accuracy(13.8 cm) of ICESat/GLAS.First,some altimetry data were selected as vertical control points to reduce errors of image correlation matching during the extraction of ASTER-based DEM.Second,ice sheet altimetry data derived from ICESat were used to generate DEM ranging from 75° to 81°S because existing ASTER data do not cover this area and high density of the coverage of ICESat altimetry data.Finally,the DEM in coverage of the expedition route was produced.The analysis of result reveals that the DEM accuracy is improved significantly.The absolute vertical accuracy of DEM is higher than 15 m in some cases and higher than 30 m for all the areas along the expedition route except from the 009-001 scene;the interior accuracy is higher than 15 m and higher than 7 m in some cases.It can meet the requirements of topographic map at 1:50000 scale,which is an economic and advantageous method to produce the topographic products. | E DongChen1,3,SHEN Qiang2,XU Ying1,3 & CHEN Gang4 1 Chinese Antarctic Center of Surveying and Mapping,Wuhan University,Wuhan 430079,China 2 Institute of Seismology China Earthquake Administration,Wuhan 430071,China 3 Key Laboratory of Polar Surveying and Mapping,State Bureau of Surveying and Mapping,Wuhan University,Wuhan 430079,China 4 Department of Geography,University of Calgary,Calgary,Alberta T2N 1N4,Canada | 2009 | Science China Earth Sciences2009，52，5： | 9 |
| 10 | Comparison of Wirsung-jejunal duct-tomucosa and dunking technique for pancreatojejunostomy after pancreatoduodenectomy显示文摘Pancreato-enteric reconstruction after pancreatoduodenectomy (PD) is still a source of debate because of the high incidence of complications. Among the various types of pancreato-jejunostomies we don’t know yet which is the best in terms of anastomotic failure and related complications rates. Wirsung-jejunal duct-to-mucosa anastomosis (WJ) and 'dunking' pancreato-jejunal anastomosis (DPJ) are the two most used ones worldwide but conflicting results are reported. To determine which is the safer anastomosis and to define when an anastomosis should be preferred, we retrospectively reviewed two groups of patients who underwent WJ or DPJ. METHODS:Twenty-three patients underwent PD with WJ (n=17) with dilated (WJD) (n=9) or not-dilated Wirsung’s duct (WJND) (n=8) or with a DPJ (n=6) over a 3-year period at a single institution. RESULTS: The complications rate was high in all groups of patients (33.3% in WJD, 37.5% in WJND and 66.7% in DPJ). A pancreatic fistula developed in one patient in each group (11. 1% in WJD, 12. 5% in WJND and 16. 7% in DPJ). All these patients were managed conservatively. Anastomotic disruption took place in the WJ patients especially in the WJND group (n=2) compared to the WJD (n=1) (25% vs 11.1%) or DPJ groups (0%) : these three patients required a re-operation. Overall, the anastomotic defects were higher in patients who underwent WJND (37.5%), compared to WJD (22.2%) and to DPJ (16.7%). However, no statistical differences were found among the groups. Delayed gastric emptying (DGE) and total parenteral nutrition (TPN) along with anastomotic defects were responsible for a prolonged hospital stay. CONCLUSIONS:Our results were not able to demonstrate any statistical difference between the two different techniques in preventing anastomotic failure. WJ can represent a valid choice in case of a dilated duct and a firm, fibrotic enlarged gland that could not be properly invaginated in a small jejunal loop. DGE may occur in those patients who experienced an anastomotic failure and required a TPN regimen with a prolonged hospital stay. | Unita di Chirurgia, Dipartimento di Fisiopatologia Clinica (Batignani G, Fratini G, Zuckermann M and Tonelli F) and Dipartimento di Statistica (Bianchini E), Universitd degli Studi di Firenze, Flo rence, Italy | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，3： | 9 |
| 11 | Study on expresion of adherent proteins related to regulation of hematopoiesis in long-term cultured human bone marrow stromal cells显示文摘Studyonexpresionofadherentproteinsrelatedtoregulationofhematopoiesisinlongtermculturedhumanbonemarrowstromalcels.LiuJiewen,... | Liu Jiewen, Qi Shuling, E de Wynter, et al. Institute of Hematology, CAMS and PUMC, Tianjin 300020, China. | 1998 | Chinese Medical Journal1998，，2： | 8 |
| 12 | Quantum phase transition and entanglement in Li atom system显示文摘By use of the exact diagonalization method, the quantum phase transition and en- tanglement in a 6-Li atom system are studied. It is found that entanglement appears before the quantum phase transition and disappears after it in this exactly solvable quantum system. The present results show that the von Neumann entropy, as a measure of entanglement, may reveal the quantum phase transition in this model. | SI LiMing1 & HOU JiXuan2 1 Department of Electronic Engineering, School of Information Science and Technology, Beijing Institute of Technology, Beijing 100081, China 2 Laboratoire de Chimie, UMR 5182 CNRS, Ecole Normale Supérieure de Lyon, 46, Allée d’Italie, F-69364 Lyon Cedex 07, France | 2008 | Science China(Physics,Mechanics & Astronomy)2008，51，11： | 6 |
| 13 | N_2O emissions from agricultural soils in the North China Plain: the effect of chemical nitrogen fertilizer and organic manure显示文摘An enclosed chamber technique was used to measure N 2O emissions from intensively agricultural soils of the North China Plain during the periods of 1995—1996 and 1997—1998, to reflect distinct components of winter wheat and summer maize growing seasons. The results showed that the continuous application of fertilizer in agricultural soils increased N\-2O emissions by a factor of 24.1—28.1, the calculated annual chemical N fertilizer\|transformed N\-2O\|N emissions was 0.67%. Our results indicated that the application of organic manure also had a significant influence on soil N 2O emissions, which combined with the use of chemical N increased about 20% in a year. It was calculated that there were about 0.11% N of organic manure transformed as N 2O N. Annual mean N 2O emission from our study area of fertilized soils was estimated to be 57.1 μgN 2O/(m 2·h). A weak correlation was also found between N 2O emissions and soil available nitrogen content NH + 4. | DONG Yun\|she 1,2,3 , Dieter Scharffe 2, Manfred Domroes 3, QI Yu chun 1, ZHANG Shen 1 (1. Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China E\|mail: dongys@dls.iog.ac.cn 2. M | 2000 | Journal of Environmental Sciences2000，12，4： | 6 |
| 14 | Analysis of genotype differences of rice response to low Zn^(2+) activity and some morphological characteristics显示文摘Zinc deficiency is one of the most widespread micro-nutritional disorder for rice. To solve the problem, screening Zn-efficient cuivivars is an available method and understanding genotype difference of Zn efficiency and their morphological and physiological characteristics is important. | WANG Renmin, Dept of Agro YANG Xiao’e and YANG Yuai, Dept of Agri Chemistry, ZheJiang Agri Univ, Hangzhou 310029, China | 1998 | Chinese Rice Research Newsletter1998，6，3： | 5 |
| 15 | Comparison of physiological responses to oxidative and heavy metal stress in seedlings of rice paddy, Oryza sativa L.显示文摘Physiological responses on the bases of activities of antioxidant enzymes: peroxidase, catalase, superoxide dismutase and glutathione reductase as well as estimation of total protein, lipid peroxidation and thiols in the form of protein, non\|protein, glutathione and phytochelatin measured in growing seedlings of paddy, Oryza sativa L., from day 2 to 8 were compared following treatment of seeds for 5h with oxidative agents, paraquat 5 × 10 -5 , 10 -4 , 10 -3 mol/L, H 2O 2 10 -3 , 5×10 -3 , 10 -2 mol/L, and CdCl 2 10 -5 , 10 -4 , 5×10 -3 mol/L. A significant induction of all antioxidant enzymes along with an increase in the levels of protein, lipid peroxidation and glutathione was noted in response to oxidative stress, CdCl 2 induced significant peroxidase and catalase activities but not superoxide dismutase. In a marked contrast from oxidative stress, CdCl 2 decreased glutathione reductase activity as well as glutathione levels but increased phytochelatin level. The different physiological responses thus underlined the crucial involvement of glutathione and phytochelatin in the oxidative and heavy metal\|linduced adaptive responses respectively. | YU Guo\|ying, WANG Guo\|ping, HE Chi\|quan (Lab for Wetland Process and Environment, Changchun Institute of Geography, The Chinese Academy of Sciences, Changchun 130021,China E\|mail: yuguoying@mail.ccig.ac.cn) | 2000 | Journal of Environmental Sciences2000，12，4： | 5 |
| 16 | HIGH-ENERGY IONS PRODUCED IN EXPLOSIONS OF SUPERHEATED ATOMIC CLUSTERS显示文摘 | T Ditmire J W G Tisch E Springate M B Mason N Hay R A Smith J Marangos and M H R Hutchinson | | 0，，： | 4 |
| 17 | Effect of garlic and garlic-green tea mixture on serum lipids in MNNG-induced experimental gastriccarcinoma and precancerous lesion显示文摘EfectofgarlicandgarlicgreenteamixtureonserumlipidsinMNNGinducedexperimentalgastriccarcinomaandprecancerouslesionSUQi,LUOZh... | SU Qi, LUO Zhao Yang, TENG Hua, YUN Wei Dong, Li Yi Qing and HE Xin E Institute of Oncology, Hengyang Medical College, Hengyang 421001, Hunan Province, China | 1998 | World Journal of Gastroenterology1998，4，1： | 4 |
| 18 | Landscape ecological characteristics in temporal changes of riverside open space in urbanized area显示文摘1IntroductionRecentlyinJapan,theriverspaceinurbanareasischangingintoriverparks,riveropenspaceandnatureorientedbank.Consequen... | Shigeru TANIMOTO *, Nobukazu NAKAGOSHI Graduate School for International Development and Cooperation, Hiroshima University, Kagamiyama, Higashi Hiroshima 739 8529, Japan. E mail: shigeru.tanimoto@aratani.co.jp | 1999 | Journal of Environmental Sciences1999，11，2： | 3 |
| 19 | Production of citric and oxalic acids and solubilization of calcium phosphate by Penicillium biaii显示文摘 | Cunningham J E and Kuiacy C | 1992 | Appl Env- iron Micro1992，58，5： | 3 |
| 20 | Eco-environment evolvement analysis of Ertan reservoir catchment based on remote sensing显示文摘Hydropower project may bring with it social-economic profits as well as side effects.The built dam and reservoir often cause some problems to the surrounding areas,among which the ecological and environmental effects caused by hydropower projects are always concerned by the public.In this article,we take the Ertan reservoir catchment as the research area and try to quantitatively analyze the variation of vegetation cover and soil erosion by remote sensing technique,and to comprehensively assess the evolvement and development trend of reservoir catchment.Soil erosion,land use/cover are used as ecological and environmental indicators which reflect the changes before,after and in the period of the construction of Ertan hydropower station.Supported by the multi-source remote sensing data(from satellite Landsat and CBERS) and DEM data,the land use/cover is interpreted through RS images which are classified both by unsupervised and supervised method,and the driving factors of the ecological changes are also analyzed.At the same time,the changes of soil loss are also monitored and analyzed during flood seasons of Ertan reservoir area before and after reservoir impoundment(1995,2000 and 2005) using the revised universal soil loss equation(RUSLE) .The results show that during the recent 13 years the arable land area has decreased obviously,and construction area and water surface have increased slightly.The increase of vegetation cover has some relations with the implementation of local ecological projects,i.e.,de-farming to forestry and de-farming to pasture projects.At the same time,changes may also be caused by the climate adjustment in the reservoir area.In the ten years from 1995 to 2005,the high soil loss classes were transforming to lowly level classes continuously.All of these show that the soil loss of Ertan reservoir area is getting better. | PANG ZhiGuo ,GE DeXiang & FU Jun E State Key Laboratory of Simulation and Regulation of Water Cycle in River Basin,,China Institute of Water Resources and Hydropower Research,Beijing 100048,China | 2011 | Science China(Technological Sciences)2011，54，S1： | 3 |
您所在的机构暂未开通此部分的功能，请联系管理员