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127篇 您的检索式：作者名="ARAVIN A"

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	题名	作者	年代	出处	被引量
1	Hepatocytes isolated from neoplastic liver-immunomagnetic purging as a new source for transplantation显示文摘AIM: To investigate whether hepatocytes isolated from macroscopically normal liver during hepatic resection for neoplasia could provide a novel source of healthy hepatocytes, including the development of reliable protocols for malignant cells removal from the hepatocyte preparation. METHODS: Hepatocytes were procured from resected liver of 18 patients with liver tumors using optimised digestion and cell-enrichment protocols. Suspensions of various known quantities of the HT-29 tumor cell line and patient hepatocytes were treated or not with Ep-CAM-antibody-coated immunomagnetic beads in order to investigate the effi cacy of tumor-purging by immunomagnetic depletion, using a semi-quantitative RT-PCR method developed to detect tumor cells. Immunomagnetic bead-treated or bead-untreated tumor cell-hepatocyte suspensions were transplanted intra-peritoneally in Balb/C nude mice to assess the rates of tumor development. RESULTS: Mean viable hepatocyte yield was 9.3 x 106 cells per gram of digested liver with mean viability of 70.5%. Immunomagnetic depletion removed tumor cells to below the RT-PCR detection-threshold of 1 tumor cell in 106 hepatocytes, representing a maximum tumor purging efficacy of greater than 400 000-fold. Transplanted, immunomagnetic bead-purged tumor cell-hepatocyte suspensions did not form peritonealtumors in Balb/C nude mice. Co-transplantation of hepatocytes with tumor cells did not increase tumorigenesis of the tumor cells. CONCLUSION: Immunomagnetic depletion appears to be an effective method of purging contaminating tumor cells to below threshold for likely tumorigenesis. Along with improved techniques for isolation of large numbers of viable hepatocytes, normal liver resected for neoplasia has potential as another clinically useful source of hepatocytes	Aravin Gunasegaram Javed Akhter Peng Yao Loreena A Johnson Stephen M Riodan David L Morris	2008	World Journal of Gastroenterology2008，14，32：	2
2	Human microRNA targets显示文摘	John B Enright AJ Aravin A	2004	PLoS Biol2004，2，11：	1
3	A novel class of small RNAs binds to MILl protein in mouse testes显示文摘	Aravin A Gaidatzis D Pfeffer S	2006	Nature2006，442，7099：	1
4	Clustering and conservation patterns of human microRNAs显示文摘	Altuvia Y Landgraf P Lithwick G Elefant N Pfeffer S Aravin A Brownstein M J Tuschl T and Margalit H		0，，08：	1
5	Identification and characterization of small RNAs involved in RNA silencing显示文摘	Aravin A Tuschl T	2005	FEBS Lett2005，579，26：	1
6	Human microRNA targets显示文摘	Bino J Enright AJ Aravin A	2004	PLoS Biol2004，2，11：	1
7	Discrete small RNA-generating loci as master regula- tors of transposon activity in Drosophila 显示文摘	BRENNECKE J ARAVIN A A STARK A	2007	Cell2007，128，6：	1
8	Human miRNA targets显示文摘	John B Enright A J Aravin A	2004	PLos Biol2004，2，：	1
9	Discrete small RNA generating loci as master regula- tors of transposon activity in Drosophila 显示文摘	BRENNECKE J ARAVIN A A STARK A	2007	Cell2007，128，6：	1
10	Human microRNA targets显示文摘	John B Enright A J Aravin A	2004	PLoS Biol2004，2，11：	1
11	Identification and characterization of small RNA s involved in R NA silencing显示文摘	Aravin A Tuschl T	2005	FEBS Lett2005，579，26：	1
12	A novel class of small RNAs bind to MILI protein in mouse testis 显示文摘	Aravin A Gaidatzis D Pfeffer S	2006	Nature2006，442，7099：	1
13	Human MicroRNA targets显示文摘	John B Enright AJ Aravin A	2004	pros Biol2004，2，11：	1
14	Human Micro RNA targets显示文摘	John B Enright AJ Aravin A	2004	PLo S Biology2004，2，11：	1
15	Human microRNA targets 显示文摘	John B Enright A Aravin A	2004	PLoS Siol2004，2，11：	1
16	Human microRNA targets显示文摘	John B Enright AJ Aravin A	2004	PLoS Biology2004，2，11：	1
17	Human microRNA targets 显示文摘	John B Enright AJ Aravin A et aI	2004	PLoS Bioi2004，2，：	1
18	A novel class of small RNAs bind to MILI protein in mouse testes显示文摘	Aravin A Gaidatzis D Pfeffer S	2006	Nature2006，442，7099：	1
19	Discrete small RN A-generating loci as master regulators of transposon acti'dty in Drosophila显示文摘	BRENNECKE J Aravin A A Stark A	2007	Cell2007，128，：	1
20	Human Micro RNA targets显示文摘	John B Enright AJ Aravin A	2004	PLo S Biol2004，2，11：	1

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